This Article  • References  • Full text PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

DORIS DONAHUE, BS; RUTH GUSSEN, MD

Arch Otolaryngol. 1966;83(1):28.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

CELLOIDIN embedding of human temporal bones is a time-consuming process because of the size and complexity of the specimens. The temporal bone contains numerous air spaces and delicate structures which are often not completely infiltrated by the usual methods of embedding.

The present study was undertaken to develop a rapid and more efficient method of celloidin embedding of human temporal bones.

Materials and Methods

Eight human temporal bones, averaging 28 gm in weight and 4.3 by 3.6 by 3.0 cm in dimension, were fixed in 10% neutral buffered Formalin and decalcified in tetrasodium edetate at neutral pH as described by Gussen and Donahue.¹ The end point of decalcification was determined by x-ray. The specimens were then run through graded alcohols to absolute alcohol-ether (1:1) in an autotechnicon for eight hours in each solution. The slow oscillating motion of the autotechnicon insured thorough dehydration of the specimens. Parlodion was chosen . . . [Full Text PDF of this Article]

Author Affiliations
LOS ANGELES

From the Otologic Research Laboratory and the Division of Laboratories, Cedars of Lebanon Division, Cedars-Sinai Medical Center.

Footnotes
Submitted for publication June 16, 1965.

Reprint requests to 4833 Fountain Ave, Los Angeles, Calif 90029 (Dr. Gussen).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?