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Itzhak Brook, MD, MSc

Arch Otolaryngol Head Neck Surg. 2005;131:1017-1019.

ABSTRACT
Objective  To evaluate the organisms recovered from infected sinuses and associated intracranial abscesses (IAs).

Design  Retrospective review of findings from aspirate of pus from 10 infected sinuses and their corresponding IAs.

Setting  Academic medical center.

Patients  Ten patients diagnosed as having sinusitis (age range, 7-58 years).

Main Outcome Measure  Aerobic and anaerobic bacteria findings from infected sinuses and IAs.

Results  Polymicrobial flora was found in 9 sinuses and 8 IAs. Anaerobes were isolated from all sinuses and 9 IAs. A total of 26 isolates (2.6 isolates per specimen) were recovered from the sinuses: 19 anaerobic, 6 aerobic or facultative, and 1 microaerophilic; 17 isolates were found in the IAs (1.7 isolates per site): 13 anaerobic, 2 aerobic or facultative, and 2 microaerophilic. The predominant anaerobes were Fusobacterium species (in 5 corresponding sinuses and abscesses, 1 in a sinus only, and 1 in an IA only), Prevotella species (in 3 corresponding sinuses and abscesses), Peptostreptococcus species (in 2 corresponding sinuses and abscesses, and 4 in a sinus only), Staphylococcus aureus, Haemophilus influenzae type b, microaerophilic streptococci, and Bacteroides ureolyticus (in 1 corresponding sinus and abscess each). Streptococcus pneumoniae was recovered 2 times, only from a sinus. -Hemolytic streptococci and -hemolytic streptococci group F were each isolated once from the sinus. Concordance in the microbiological findings between the sinus and the IA was found in all instances. However, certain organisms were present at only one or the other site.

Conclusion  These data illustrate the concordance in the recovery of organisms from infected sinuses and their associated IA and confirm the importance of anaerobic bacteria in sinusitis and IA.

INTRODUCTION

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Intracranial abscess (IA) is a rare but serious complication of paranasal sinusitis.^1-2 Because of its life-threatening nature, IA must be managed as a medical and surgical emergency. Recognition of the potential pathogens and their origin can assist in the proper management of the infection.

Most studies have investigated the microbiology of IA^3-5 and sinusitis^6-8 separately and found it to be monomicrobial most of the time and occasionally polymicrobial due to aerobic and anaerobic bacteria. However, the role of anaerobic bacteria in IA has not been well studied in most reports because the methods for recovery of anaerobes were inadequate or not consistently used.^9-11

To my knowledge, the unique microbiology of sinusitis associated with IA and the correlation between the organisms at both sites has not been reported. This report describes my experience during a 26-year period studying the aerobic and anaerobic microbiology of 10 IAs and corresponding sinusitis.

METHODS

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The 10 patients included in the present report were observed between June 1977 and June 2003. Their ages ranged from 7 to 58 years, and 6 were male (Table). Antimicrobial agents were given to 3 patients prior to drainage: amoxicillin to patients 2 and 14, erythromycin to patient 9.

View this table: [in this window] [in a new window] Table. Bacteriology and Clinical Features of Sinusitis and Associated Intracranial Abscesses in 10 Patients

Cultures of sinuses and IAs were obtained during surgery using aseptically performed puncture and aspiration prior to surgical drainage. The material was collected either by syringe that was immediately sealed and transported to the laboratory within 30 minutes or by a swab that was dipped into the pus and introduced into an anaerobic transport system (Port-A-Cul; BBL Microbiology Systems, Cockeysville, Md) and generally transported to the laboratory within 2 hours.

Anaerobic bacteria material was plated onto 1 of the following: (1) prereduced Brucella blood agar enriched with phytonadione (vitamin K₁); (2) anaerobic blood agar containing colistin and nalidixic acid; or (3) enriched thioglycolate broth (containing hemin and vitamin K₁). It was then incubated inside GasPak jars (BBL Microbiology Systems) and examined at 48 and 96 hours. Plates that showed growth were maintained until the organisms were processed and identified. All cultures that showed no growth were kept for at least 7 days to verify that no growth occurred. Anaerobes were identified by techniques described previously.⁸

For aerobic bacteria, sheep blood (5%), chocolate, and MacConkey agar plates were inoculated at 37°C aerobically (MacConkey) and under 5% carbon dioxide (blood and chocolate) and examined at 24 and 48 hours. Aerobic bacteria were identified by conventional methods.⁸ -Lactamase activity was determined by use of the chromogenic cephalosporin analog 87/312 method.¹²

RESULTS

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Polymicrobial flora was found in 9 sinuses and 8 IAs, and the number of isolates ranged from 1 to 4. Anaerobic bacteria were isolated from all sinuses and 9 IAs (Table). These anaerobes (including microaerophilic streptococci) were the sole bacterial isolates in 5 sinuses (patients 2, 6, 7, 8, and 9) and 7 IAs (patients 1, 2, 4, 6, 7, 8, and 9). Concordance in the microbiological findings between the sinus and the IA was found in all instances. However, certain organisms were present only at one site and not at the other sites.

A total of 26 isolates (2.6 isolates per specimen) were recovered from the sinuses: 19 anaerobic, 6 aerobic or facultative, and 1 microaerophilic; 17 isolates were found in the IAs (1.7 isolates per site): 13 anaerobic, 2 aerobic or facultative, and 2 microaerophilic. The predominant anaerobic isolates were Fusobacterium species (in 5 corresponding sinuses and abscesses, 1 in a sinus only, and 1 in an IA only); Prevotella species (in 3 corresponding sinuses and abscesses); Peptostreptococcus species (in 2 corresponding sinuses and abscesses and 4 in a sinus only); and Staphylococcus aureus, Haemophilus influenzae type b, microaerophilic streptococci, and Bacteroides ureolyticus (in 1 corresponding sinus and abscess each). Streptococcus pneumoniae was recovered 2 times from a sinus only. -Hemolytic streptococci and -hemolytic streptococci group F were each isolated once from a sinus.

Twelve -lactamase–producing organisms were present in 6 sinuses and 6 IAs. These were Fusobacterium nucleatum (4 isolates), Prevotella species (4), S aureus (2), and H influenzae type b (2).

COMMENT

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This study illustrates the importance of anaerobic bacteria in IAs and their predominance in the associated sinusitis-affected sinus.² Our findings confirm the observation of Herrmann and Forsen,¹³ who recovered aerobic and anaerobic polymicrobial flora from 2 infected sinuses and their associated intracranial complication sites. Anaerobic bacteria were previously recovered from chronically infected sinuses.^7-8 Although several aerobic bacteria such as Streptococcus species, S aureus, and H influenzae were isolated in several instances, the recovery of mainly anaerobic bacteria from all of our patients suggests the chronic nature of their infection.

Anaerobic and microaerophilic cocci and gram-negative and gram-positive anaerobic bacilli are the most important isolates recovered from brain abscesses. These include Bacteroides, Prevotella, Fusobacterium, and Clostridium species.^3-5,13-16 The variations in collection techniques, culturing for strict anaerobes, and improper specimen handling to prevent contamination may account for differences between studies in the final organism identification.

Certain organisms such as S pneumoniae and Peptostreptococcus species were only or mainly present at the sinus and not the IA. The lower number of organisms per specimen recovered from IAs than from sinuses (1.7 vs 2.6 isolates per specimen) suggests that not all the bacteria present in the sinus cavity are able to reach the intracranial space or participate in the IA.

Past studies have not found significant correlation between sinus cultures and IAs.^9-11 However, most of the sinus cultures were done using an endoscopic method, which can lead to contamination of specimens, and methods adequate for the recovery of anaerobic bacteria were not used.

Although surgical drainage is of primary importance, administration of antimicrobial therapy is an essential part of the treatment of patients with sinusitis and IA and other related complications. A growing number of anaerobic gram-negative bacilli (eg, pigmented Prevotella and Fusobacterium species) have acquired resistance to penicillin through the production of the enzyme -lactamase.¹⁷ This has also been observed in the present report, where 6 each of the sinuses and IAs contained such organisms.

The isolation of polymicrobial aerobic and anaerobic flora in most of our patients suggests their important role in sinusitis and the associated IA. However, further prospective studies are warranted that include larger numbers of patients to evaluate the concurrent prevalence of these organisms in sinusitis and the associated IAs.

AUTHOR INFORMATION

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Correspondence: Itzhak Brook, MD, MSc, 4431 Albemarle St NW, Washington, DC 20016 (ib6{at}georgetown.edu).

Submitted for Publication: June 10, 2005; accepted July 13, 2005.

Financial Disclosure: None.

Author Affiliations: Department of Pediatrics, Georgetown University School of Medicine, Washington, DC.

REFERENCES

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1. Skau NK, Nielsen KO, Osgaard O, Molgaard IL, Peitersen E. Intracranial and orbital complications of frontal and ethmoidal sinusitis. Acta Otolaryngol Stockh. 1984;412(suppl):91-94. 2. Brook I, Friedman E, Rodriguez WJ, Controni G. Complications of sinusitis in children. Pediatrics. 1980;66:568-572. FREE FULL TEXT 3. Yogev R, Bar-Meir M. Management of brain abscesses in children. Pediatr Infect Dis J. 2004;23:157-159. WEB OF SCIENCE | PUBMED 4. Calfee DP, Wispelwey B. Brain abscess. Semin Neurol. 2000;20:353-360. FULL TEXT | WEB OF SCIENCE | PUBMED 5. Brook I. Microbiology and management of brain abscess in children. J Pediatr Neurol. 2004;2:125-130. 6. Wald ER, Milmore GJ, Bowen AD, Ledema-Medina J, Salamon N, Bluestone CD. Acute maxillary sinusitis in children. N Engl J Med. 1981;304:749-754. ABSTRACT 7. Nord CE. The role of anaerobic bacteria in recurrent episodes of sinusitis and tonsillitis. Clin Infect Dis. 1995;20:1512-1524. WEB OF SCIENCE | PUBMED 8. Brook I. Bacteriology of chronic maxillary sinusitis in adults. Ann Otol Rhinol Laryngol. 1989;98:426-428. WEB OF SCIENCE | PUBMED 9. Gallagher RM, Gross CW, Phillips CD. Suppurative intracranial complications of sinusitis. Laryngoscope. 1998;108:1635-1642. FULL TEXT | WEB OF SCIENCE | PUBMED 10. Giannoni CM, Stewart MG, Alford EL. Intracranial complications of sinusitis. Laryngoscope. 1997;107:863-867. FULL TEXT | WEB OF SCIENCE | PUBMED 11. Giannoni C, Sulek M, Friedman EM. Intracranial complications of sinusitis: a pediatric series. Am J Rhinol. 1998;12:173-178. WEB OF SCIENCE | PUBMED 12. O’Callaghan CH, Morris A, Kirby SM, Shingler AH. Novel method for detection of beta-lactamase by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972;1:283-288. FREE FULL TEXT 13. Herrmann BW, Forsen JW Jr. Simultaneous intracranial and orbital complications of acute rhinosinusitis in children. Int J Pediatr Otorhinolaryngol. 2004;68:619-625. FULL TEXT | WEB OF SCIENCE | PUBMED 14. Ayyagari A, Pancholi VK, Kak VK, et al. Bacteriological spectrum of brain abscess with special reference to anaerobic bacteria. Indian J Med Res. 1983;77:182-186. WEB OF SCIENCE | PUBMED 15. Chun CH, Johnson JD, Hofstetter M, Raff M. Brain abscess: a study of 45 consecutive cases. Medicine (Baltimore). 1986;65:415-431. FULL TEXT | PUBMED 16. Aebi C, Kaufman F, Schaad UB. Brain abscess in childhood—long-term experience. Eur J Pediatr. 1991;150:282-286. FULL TEXT | WEB OF SCIENCE | PUBMED 17. Brook I, Calhoun L, Yocum P. Beta-lactamase–producing isolates of Bacteroides species from children. Antimicrob Agents Chemother. 1980;18:164-166. FREE FULL TEXT

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