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Incidence of Occult Cerebrospinal Fluid Fistula During Paranasal Sinus Surgery
Gregor Bachmann, MD;
Uta Djenabi, CandMed;
Markus Jungehülsing, MD;
Hela Petereit, MD;
Olaf Michel, MD
Arch Otolaryngol Head Neck Surg. 2002;128:1299-1302.
ABSTRACT
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Objective To determine the incidence of occult cerebrospinal fluid fistulas after endoscopic paranasal sinus surgery.
Design Prospective diagnostic test study with a 6-month follow-up in case of cerebrospinal fluid detection.
Setting Tertiary care hospital.
Subjects The study population comprised 69 patients undergoing routine endoscopic paranasal sinus surgery. Patients with an obvious intraoperative or postoperative cerebrospinal fluid fistula were not included.
Intervention Analysis of 112 samples from intraoperative applied tamponades and of 69 serum samples using a nephelometric research assay for  -trace protein (prostaglandin D synthase).
Main Outcome Measures Incidence of occult cerebrospinal fluid fistula during endoscopic paranasal sinus surgery as indicated with the help of a test for -trace protein; at least a 6-month follow-up of patients with an occult cerebrospinal fluid fistula; and relation of occult cerebrospinal fluid fistula with surgical experience of the surgeon.
Results -Trace protein was found in ethmoid roof samples from 2 patients, giving an incidence of 2.9% for occult cerebrospinal fluid fistula. Both patients were operated on by very experienced surgeons. Signs of a cerebrospinal fluid fistula were not found at follow-up at least 6 months after surgery.
Conclusions Nephelometric -trace protein assay is a highly sensitive method to detect otherwise unobserved cerebrospinal fluid fistulas. The clinical course of the 2 patients with an occult cerebrospinal fluid fistula indicated the possibility of an uneventful follow-up of patients with small fistulas.
INTRODUCTION
PATIENTS WITH a cerebrospinal fluid (CSF) fistula are at risk to develop meningitis, which is still a life-threatening condition. With a traumatic CSF leak, the cumulative risk to develop meningitis exceeded 85% after 10 years, and, in spite of extensive antibiotic treatment, the mortality rate of bacterial meningitis was reported to be 25% to 50%.1-3 Cerebrospinal fluid fistulas occur as congenital defects, after head trauma and surgery, because of tumor invasion, or without any obvious reason.4-5 A rare cause of CSF fistula are bony defects due to arachnoid granulation tissue.6 Cerebrospinal fluid leaks are a well-known complication of paranasal sinus surgery. However, the incidence of an unobserved, intraoperative CSF fistula—termed occult CSF fistula—is not presently known. Furthermore, it is not known whether an occult CSF fistula will finally lead to a clinical obvious permanent fistula.
Recently, a new nephelometric research assay was introduced for the quantification of -trace protein (-TP), which is an ideal immunological marker for the detection of traces of CSF fluid. -Trace protein belongs to the lipocalin family of proteins with carrier and enzymatic functions and is produced in the meninges and choroid plexus and, to a lesser extent, in astrocytes.7 The physiological role of the protein is not known. In 1993, after the determination of amino acid sequence, it was shown that -TP is identical with prostaglandin D synthase (EC 5.3.99.2, as classified by the Enzyme Commission).8 -Trace protein showed a CSF/serum ratio of 33, which is the highest of the CSF-specific proteins presently known .9 For the detection of CSF fistulas, -TP showed high predictive values in 98 subjects.10 The aims of the present study were to present a new -TP nephelometric research assay as a tool for clinical research and to investigate the incidence of occult CSF fistula in patients undergoing routine paranasal sinus surgery in a prospective setting.
METHODS
PATIENTS AND SURGICAL PROCEDURE
Sixty-nine patients were enrolled between 1998 and 2000. The mean ± SD age was 47.9 ± 13.0 years, and the sex distribution was 27.5% women and 72.5% men. Patients with previous surgery and patients with fungal paranasal sinus disease were included. Patients with a clearly visible CSF leakage during surgery were not included. The indication for surgery was chronic paranasal sinus disease with or without nasal polyps. All patients underwent endoscopic paranasal sinus surgery according to the Messerklinger or Wigand technique.11-15 Thirteen patients underwent unilateral procedures. Bilateral procedures were performed in 56 patients. Highly and less experienced surgeons were included, but all surgeons had at least 2 years of otorhinolaryngologic surgical training.
SAMPLING
Raucocel (Rauscher, Germany) sinus packs were placed at the ethmoid roof at the end of the surgical procedure. After the Raucocel packs were inserted, they were soaked with doxycycline solution to prevent local postoperative infection. At the first postoperative day, a serum sample was taken, and at the second postoperative day, the sinus packs were removed. Fluid samples were obtained by pressing the sinus packs. The samples were centrifuged at 4000 rpm for 5 minutes and were kept at -40°C before investigating for -TP nephelometry.
FOLLOW-UP
Patients with a positive or a suggestive -TP result were followed up at least 6 months after surgery. A nasal endoscopy was performed, and a sinus pack tamponade was used to collect a fluid sample. Under local anesthesia, sinus packs were placed at the ethmoid roof on both sides. The sinus packs were left in place for 3 hours. After removal, the sinus packs were pressed, and the fluid samples were analyzed for -TP.
-TP NEPHELOMETRIC ASSAY
For quantitative determination of -TP, a newly developed nephelometric research assay, N Latex -TP (Dade Behring, Liederbach, Germany), was used. N-Latex -TP is a lyophilized reagent for the Behring Nephelometer Analyzer (Dade Behring). Polystyrene particles coated with immunoaffinity-purified polyclonal antibodies from rabbit against human -TP were agglutinated in the presence of -TP. The increase in light scattering caused by agglutination was measured by laser absorption as described elsewhere.16 The sample volume was 5 µL. The analytical imprecision of the assay was 2.3% to 6.5%. The detection limit for the undiluted sample was 2.5 µg/L.17 The nephelometric assay offers results after 20 minutes.
In accordance with recently published data, samples with a -TP concentration of 6 mg/L or higher were reported as positive, indicating the presence of CSF. A result lower than 3 mg/L was defined as negative for CSF traces. A result between 3 and 6 mg/L was suggestive of CSF.
CALCULATION OF CORRECTED -TP RESULTS
Kleine and coworkers17 found that a small amount of -TP binds to sinus pack materials. Because of this, the results might be up to 30% lower compared with samples, which were collected without sinus packs. Therefore, the -TP results from all Raucocel sinus packs were corrected using the formula x = (y x 100):70.
RESULTS
The quantitative data are given in Table 1. Calculating the incidence in 69 subjects, we found an occult CSF fistula in 2.9%. When calculating the incidence on the basis of 112 sinus packs, we found an occult CSF fistula in 2.7% (3 positive samples).
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Quantitative Data of Serum Samples and Sinus Pack Samples*
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The average value of -TP in serum was 0.69 ± 0.33 mg/L. As usual, all sinus packs contained a large amount of serum, blood, and doxycycline solution. Three samples from 2 patients showed elevated -TP results. Also, when taking into account that up to 30% of -TP was bound to the sinus pack material, the results of the sinus packs showed presence of CSF in the same 2 cases.
CASE 32
A 64-year-old patient underwent a revision paranasal sinus surgery because of recurrence of polyposis nasi. The bony structures showed no anatomic malformation, as demonstrated by a high-resolution computed tomographic scan of the coronal plane. Surgery was performed under general anesthesia by one of the most experienced surgeons of the department. The procedure was in accordance with the Wigand technique. The intraoperative and postoperative course was unremarkable. There were no headaches, no fever, and no signs of rhinorrhea. Analysis of samples from the sinus packs for -TP indicated clear evidence of CSF on both sides. Therefore, the patient was followed up 9 months later. The patient had no complaints. He did not develop meningitis, and signs of rhinorrhea were not found. The -TP results at follow-up were 0.5 and 0.3 mg/L, indicating no traces of CSF.
CASE 33
A 53-year-old patient presented with polyposis nasi. She had no previous surgery. Again, surgery was performed by one of the very most experienced surgeons of the department under general anesthesia. The fluid samples from the paranasal sinus packs showed -TP values of 3.1 and 1.7 mg/L, suggesting the presence of CSF on one side. According to the study protocol, the patient was followed up 6 months after surgery. The patient did not have any complaints, and she did not develop meningitis. Signs of rhinorrhea or clinical signs of a CSF fistula were not present, and the -TP results of the sinus packs at follow-up were not suggestive for the presence of CSF traces.
COMMENT
The verification of a CSF fistula can be difficult because of several aspects. Patients with a CSF fistula might present without clinical symptoms. It is possible that CSF fistulas recur or become obvious, causing bacterial meningitis decades after a head trauma.18-21 The clinical sign of rhinorrhea is not frequently present because minute amounts of CSF can flow intermittently through a small fistula. Therefore, sensitive, inexpensive, and, if possible, noninvasive methods to detect traces of CSF are warranted. Once a CSF leak is diagnosed, fistulas are closed by an otorhinolaryngological approach with high success rates.22-27
At present, a gold standard to confirm a CSF fistula is lacking.28 The glucose test is outdated and not recommended any more.29 High-resolution computed tomography of the paranasal sinuses and petrous bone or flow-sensitive magnetic resonance imaging offer information about the localization of a CSF fistula, but are not suitable as screening methods.30 Computed tomographic cisternography, 111In-DTPA (indium III diethylenetriamine pentaacetic acid) radionuclide cisternography, and visualization of CSF using sodium fluorescein are all invasive methods and not suitable for a prospective clinical study.31 The 2-transferrin assay is available only at a few centers and requires a laboratory workup of at least 3 hours.32-33 Data regarding sensitivity vary between 0.9 and 0.79.34-35 Other laboratory methods to detect CSF traces failed to gain clinical relevance. The electronic nose offers a new method to differentiate between serum and CSF, but it is unclear whether it is possible to detect CSF traces in serum samples.36
We chose the new -TP assay because it offers high predictive values in the detection of CSF fistulas.10 Also, small sample volumes are sufficient, and the method is not invasive. The costs are low, and the results are available after 20 minutes when using the nephelometric assay. Furthermore, quantitative data are well suited for a screening test.
A prospective study investigating the incidence of CSF fistula after endoscopic paranasal sinus surgery is lacking at present. From a retrospective study, the incidence of postoperative CSF fistulas was reported to be less than 1% in 800 patients.37 Solomon et al38 investigated fluid samples after endoscopic sinus surgery and after neck dissection in 18 subjects using 2-transferrin. They reported that all samples did not contain 2-transferrin and thus excluded the theorem of extracranial drainage of CSF.38
Sinus packs removed at the second day after surgery contain a large amount of serum and blood. It might be difficult to detect CSF traces in these packs. However, from our results, occult CSF fistulas occur during endoscopic paranasal sinus surgery with an incidence of 2.9%. We therefore believe that the -TP test is the most sensitive method to trace CSF.
The experience of the surgeon does not seem to correlate with the complication of an occult CSF fistula. In contrast, the 2 patients with an occult CSF fistula were both operated on by very experienced surgeons.
Furthermore, we could not find data in the literature giving information about the prognosis of occult CSF fistulas. The results of the present study indicate that an intraoperative occult CSF fistula might close spontaneously during the postoperative healing process. In the 2 patients with an occult CSF fistula, the postoperative course and the 6 months' follow-up were uneventful. However, the surgeon must be aware of the possibility of an occult CSF fistula, and a close, long-term follow-up of patients with a proven occult CSF fistula is advisable.
AUTHOR INFORMATION
Accepted for publication May 8, 2002.
Corresponding author: Gregor Bachmann, MD, Øre-nese-hals-avdelingen, Universitetssykehuset i Nord-Norge, N–9038 Tromsø, Norway (e-mail: gregor.bachmann{at}unn.no).
From the Department of Otorhinolaryngology, University Hospital of Northern Norway, Tromsø (Dr Bachmann); the Departments of Otorhinolaryngology–Head and Neck Surgery (Ms Djenabi and Dr Michel) and Neurology (Dr Petereit), University of Cologne Medical School, Köln, Germany; and the Department of Otorhinolaryngology, Klinikum "Ernst v. Bergmann," Potsdam, Germany (Dr Jungehülsing).
REFERENCES
| |
1. Hubbard JL, McDonald TJ, Pearson BW, Laws ER Jr. Spontaneous cerebrospinal fluid rhinorrhea: evolving concepts in diagnosis and surgical management based on the Mayo Clinic experience from 1970 through 1981. Neurosurgery. 1985;16:314-321.
ISI
| PUBMED
2. Eljamel MS, Foy PM. Acute traumatic CSF fistulae: the risk of intracranial infection. Br J Neurosurg. 1990;4:381-385.
PUBMED
3. Durand ML, Calderwood SB, Weber DJ, et al. Acute bacterial meningitis in adults: a review of 493 episodes. N Engl J Med. 1993;328:21-28.
FREE FULL TEXT
4. Har-El G. What is "spontaneous" cerebrospinal fluid rhinorrhea? classification of cerebrospinal fluid leaks. Ann Otol Rhinol Laryngol. 1999;108:323-326.
ISI
| PUBMED
5. Foyt D, Brackmann DE. Cerebrospinal fluid otorrhea through a congenitally patent fallopian canal. Arch Otolaryngol Head Neck Surg. 2000;126:540-542.
FREE FULL TEXT
6. Gacek RR, Gacek MR, Tart R. Adult spontaneous cerebrospinal fluid otorrhea: diagnosis and management. Am J Otol. 1999;20:770-776.
PUBMED
7. Blödorn B, Bruck W, Tumani H, et al. Expression of the beta-trace protein in human pachymeninx as revealed by in situ hybridization and immunocytochemistry. J Neurosci Res. 1999;57:730-734.
FULL TEXT
|
ISI
| PUBMED
8. Hoffmann A, Conradt HS, Gross G, Nimtz M, Lottspeich F, Wurster U. Purification and chemical characterization of beta-trace protein from human cerebrospinal fluid: its identification as prostaglandin D synthase. J Neurochem. 1993;61:451-456.
ISI
| PUBMED
9. Huber AR. Zur neuen Diagnostik von Liquorfisteln. HNO. 2000;48:487-488.
FULL TEXT
|
ISI
| PUBMED
10. Bachmann G, Nekic M, Michel O. Clinical experience with beta-trace protein as a marker for cerebrospinal fluid. Ann Otol Rhinol Laryngol. 2000;109:1099-1102.
ISI
| PUBMED
11. Wigand ME. Transnasal, endoscopical sinus surgery for chronic sinusitis, II: endonasal operation of the maxillary antrum. HNO. 1981;29:263-269.
ISI
| PUBMED
12. Wigand ME. Transnasal, endoscopical sinus surgery for chronic sinusitis, I: a biomechanical concept of the endonasal mucosa surgery. HNO. 1981;29:215-221.
ISI
| PUBMED
13. Wigand ME, Steiner W, Jaumann MP. Endonasal sinus surgery with endoscopical control: from radical operation to rehabilitation of the mucosa. Endoscopy. 1978;10:255-260.
FULL TEXT
|
ISI
| PUBMED
14. Messerklinger W. Background and evolution of endoscopic sinus surgery. Ear Nose Throat J. 1994;73:449-450.
PUBMED
15. Messerklinger W. Diagnosis and endoscopic surgery of the nose and its adjoining structures. Acta Otorhinolaryngol Belg. 1980;34:170-176.
PUBMED
16. Petereit HF, Bachmann G, Nekic M, Althaus H, Pukrop R. A new nephelometric assay for beta-trace protein (prostaglandin D synthase) as an indicator of liquorrhoea. J Neurol Neurosurg Psychiatry. 2001;71:347-351.
FREE FULL TEXT
17. Kleine TO, Damm T, Althaus H. Quantification of ß-trace protein and detection of transferrin isoforms in mixtures of cerebrospinal fluid and blood serum as models of rhinorrhea and otorrhea diagnosis. Fresenius J Anal Chem. 2000;366:382-386.
FULL TEXT
| PUBMED
18. Boenninghaus HG. Recurrent meningitides as sequelae of earlier skull base fractures. HNO. 1968;16:1-6.
PUBMED
19. Salca HC, Danaila L. Onset of uncomplicated cerebrospinal fluid fistula 27 years after head injury. Surg Neurol. 1997;47:132-133.
FULL TEXT
|
ISI
| PUBMED
20. Crawford C, Kennedy N, Weir WR. Cerebrospinal fluid rhinorrhoea and Haemophilus influenzae meningitis 37 years after a head injury. J Infect. 1994;28:93-97.
FULL TEXT
|
ISI
| PUBMED
21. Hadjihannas E, Ashkan K, Norris J. Untitled [Minerva]. BMJ. 2001;322:1374.
FREE FULL TEXT
22. Lanza DC, O'Brian DA, Kennedy DW. Endoscopic repair of cerebrospinal fluid fistulae and encephaloceles. Laryngoscope. 1996;106(9 Pt 1):1119-1125.
23. Weber R, Keerl R, Draf W, Schick B, Mosler P, Saha A. Management of dural lesions occurring during endonasal sinus surgery. Arch Otolaryngol Head Neck Surg. 1996;122:732-736.
FREE FULL TEXT
24. Hughes RG, Jones NS, Robertson IJ. The endoscopic treatment of cerebrospinal fluid rhinorrhoea: the Nottingham experience. J Laryngol Otol. 1997;111:125-128.
ISI
| PUBMED
25. Hegazy HM, Carrau RL, Snyderman CH, Kassam A, Zweig J. Transnasal endoscopic repair of cerebrospinal fluid rhinorrhea: a meta-analysis. Laryngoscope. 2000;110:1166-1172.
FULL TEXT
|
ISI
| PUBMED
26. Jones NS, Becker DG. Advances in the management of CSF leaks. BMJ. 2001;322:122-123.
FREE FULL TEXT
27. Costantino PD, Hiltzik DH, Sen C, et al. Sphenoethmoid cerebrospinal fluid leak repair with hydroxyapatite cement. Arch Otolaryngol Head Neck Surg. 2001;127:588-593.
FREE FULL TEXT
28. Skedros DG, Cass SP, Hirsch BE, Kelly RH. Sources of error in use of beta-2 transferrin analysis for diagnosing perilymphatic and cerebral spinal fluid leaks. Otolaryngol Head Neck Surg. 1993;109:861-864.
ISI
| PUBMED
29. Hull HF, Morrow G. Glucorrhea revisited: prolonged promulgation of another plastic pearl. JAMA. 1975;234:1052-1053.
FREE FULL TEXT
30. Sillers MJ, Morgan CE, Gammal TE. Magnetic resonance cisternography and thin coronal computerized tomography in the evaluation of cerebrospinal fluid rhinorrhea. Am J Rhinol. 1997;11:387-392.
FULL TEXT
|
ISI
| PUBMED
31. Wolf G, Greistorfer K, Stammberger H. Der endoskopische Nachweis von Liquorfisteln mittels der Fluoreszeintechnik. Laryngorhinootologie. 1997;76:588-594.
PUBMED
32. Skedros DG, Cass SP, Hirsch BE, Kelly RH. Beta-2 transferrin assay in clinical management of cerebral spinal fluid and perilymphatic fluid leaks. J Otolaryngol. 1993;22:341-344.
ISI
| PUBMED
33. Nandapalan V, Watson ID, Swift AC. Beta-2-transferrin and cerebrospinal fluid rhinorrhoea. Clin Otolaryngol. 1996;21:259-264.
ISI
| PUBMED
34. Oberascher G, Arrer E. Efficiency of various methods of identifying cerebrospinal fluid in oto- and rhinorrhea. ORL J Otorhinolaryngol Relat Spec. 1986;48:320-325.
PUBMED
35. Simmen D, Bischoff TH, Schuknecht B. Diagnosis of cerebrospinal fluid rhinorrhea. Laryngorhinootologie. 1997;76:583-587.
PUBMED
36. Thaler ER, Bruney FC, Kennedy DW, Hanson CW. Use of an electronic nose to distinguish cerebrospinal fluid from serum. Arch Otolaryngol Head Neck Surg. 2000;126:71-74.
FREE FULL TEXT
37. Stankiewicz JA. Cerebrospinal fluid fistula and endoscopic sinus surgery. Laryngoscope. 1991;101:250-256.
ISI
| PUBMED
38. Solomon P, Chen J, D'Costa M, Gilbert R, Davidson J, Johnston M. Extracranial drainage of cerebrospinal fluid: a study of beta-transferrins in nasal and lymphatic tissues. Laryngoscope. 1999;109:1313-1315.
FULL TEXT
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ISI
| PUBMED
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