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7β-Hydroxycholesterol Induces Apoptosis and Regulates Cyclooxygenase 2 in Head and Neck Squamous Cell Carcinoma
Gregor Heiduschka, MD;
Boban M. Erovic, MD;
Laurenz Vormittag, MD;
Christiane Skoda, MD;
Helga Martinek;
Markus Brunner, MD;
Klaus Ehrenberger, MD;
Dietmar Thurnher, MD
Arch Otolaryngol Head Neck Surg. 2009;135(3):261-267.
Objective To determine whether treatment with 7β-hydroxycholesterol (7β-HC) would trigger cell death in head and neck squamous cell carcinoma (HNSCC) cell lines in a dose-dependent fashion.
Design In vitro study.
Subjects The study included HNSCC cell lines SCC9, SCC25, CAL27, and FaDu.
Intervention We treated HNSCC cell lines with increasing doses of 7β-HC. Proliferation assays were performed to assess cell viability after treatment. Western blots were carried out to evaluate cyclooxygenase (COX)-1 and -2 expression levels.
Results Using proliferation assays and immunocytochemical analysis, we detected significant growth inhibition via apoptosis in 4 different HNSCC cell lines after treatment with 7β-HC (P < .001). The 50% inhibitory concentration levels were between 13.19 and 20.79 µmol/L after 72 hours. Western analysis indicated that COX-2, but not COX-1, levels were suppressed after treatment.
Conclusions Treatment with 7β-HC resulted in suppression of HNSCC growth in vitro. Our data warrant further investigations for the potential use of 7β-HC as a cytotoxic agent in head and neck cancer.
Author Affiliations: Departments of Otorhinolaryngology–Head and Neck Surgery (Drs Heiduschka, Erovic, Brunner, Ehrenberger, and Thurnher and Ms Martinek), Internal Medicine I, Division of Oncology (Dr Vormittag), and Radiotherapy and Radiobiology (Dr Skoda), Medical University of Vienna, Vienna, Austria.
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