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Increased Expression of Pigment Epithelium–Derived Factor in Allergic Rhinitis
Hee Joon Kang, MD;
Hyo-Hyun Park, MSc;
Sung Won Chae, MD;
Soon Jae Hwang, MD;
Sang Hag Lee, MD;
Seung Hoon Lee, MD;
Heung-Man Lee, MD, PhD
Arch Otolaryngol Head Neck Surg. 2008;134(10):1094-1098.
Objectives To investigate the expression of messenger RNA (mRNA) of the gene for pigment epithelium–derived factor (PEDF) (OMIM *172860) and PEDF protein and to localize the PEDF protein in the nasal mucosa of patients with allergic rhinitis and of control subjects.
Design Investigation of PEDF mRNA and PEDF protein expression in the nasal mucosa using reverse transcription–polymerase chain reaction, Western blotting, and immunohistochemical staining.
Participants We used inferior turbinate mucosal samples from 10 patients with allergic rhinitis and 10 matched healthy control subjects.
Interventions We extracted PEDF mRNA from the inferior turbinate mucosa samples and performed reverse transcription–polymerase chain reaction analysis. We used Western blotting to analyze differences in expression levels of PEDF protein between patients with allergic rhinitis and healthy controls, and the PEDF protein was localized immunohistochemically.
Results The expression levels of PEDF mRNA and PEDF protein in the nasal mucosa were significantly increased in patients with allergic rhinitis compared with those in nonallergic controls. The PEDF protein was expressed in the epithelium and submucosal glands.
Conclusions We found that PEDF protein is expressed in the human nasal mucosa, and its expression is increased in allergic rhinitis. These results suggest a possible contribution of PEDF to the chronic inflammation of the nasal mucosa in allergic rhinitis.
Author Affiliations: Department of Otorhinolaryngology–Head and Neck Surgery (Drs Kang, Chae, Hwang, Sang H. Lee, Seung H. Lee, and H.-M. Lee), and Division of Brain Korea 21 Program for Biomedical Science (Ms Park and Dr H.-M. Lee), Korea University College of Medicine, Seoul.
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