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Javier Vicente, MD, PhD; Almudena Trinidad, MD, PhD; Rafael Ramírez-Camacho, MD, PhD; José Ramón García-Berrocal, MD, PhD; Jose Ángel González-García, MD; Andres Ibáñez, MD; Maria Teresa Pinilla, MD, PhD

Arch Otolaryngol Head Neck Surg. 2007;133(6):587-592.

Objective  To develop a valid animal model for otitis media with effusion (OME).

Design  Forty specific pathogen–free Wistar rats underwent a procedure based on the permanent obstruction of pharyngeal eustachian tube by means of electrocoagulation without any manipulation.

Setting  Ear Research Group, Department of Otorhinolaryngology, Puerta de Hierro Hospital, Universidad Autonoma de Madrid, Madrid, Spain.

Main Outcome Measures  The assessment of OME by otoscopy and tympanometry. The rats were humanely killed at 15 and 90 days, and temporal bones were obtained and processed for histopathologic study.

Results  The histopathologic study of the temporal bones demonstrated the occurrence of chronic effusion and mucosal changes owing to mucoperiosteal enlargement.

Conclusions  Comparison with other experimental models was made. Our animal model was consistent and reproducible and resembled human OME.

Author Affiliations: Grupo de Investigación Otológica, Servicio de Otorrinolaringología, University Hospital Puerta de Hierro, Universidad Autonoma de Madrid, Madrid, Spain.

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