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Induction of Immunological Antitumor Effects by Adenovirus-Mediated Gene Transfer of B7-1 in a Murine Squamous Cell Carcinoma Cell Line
Yasuhiko Hoshitani, MD;
Haruhiko Ishida, MD, PhD;
Naoki Otsuki, MD, PhD;
Toshiro Shirakawa, MD, PhD;
Akinobu Gotoh, MD, PhD;
Ken-ichi Nibu, MD, PhD
Arch Otolaryngol Head Neck Surg. 2007;133(3):270-275.
Objective To evaluate the antitumor immune effects of B7-1 gene expression mediated by adenoviral vectors against squamous cell carcinoma. Transfection of the costimulatory molecule B7-1 gene into certain murine tumors increases antitumor immunity and suppresses tumor growth.
Design In vitro and in vivo study.
Interventions A murine squamous cell carcinoma cell line, KLN205, was infected with adenoviral vectors carrying either B7-1 (AdB7) or LacZ (AdCL) genes. Infected cells were injected subcutaneously into the flanks of DBA/2 mice.
Main Outcome Measures The growth of tumors infected with adenviral vectors was measured.
Results AdB7-infected cells grew significantly slower than AdCL-infected cells in vivo, while there was no significant difference in the growth rates between the 2 groups in vitro. Moreover, significant growth suppression of rechallenged noninfected parental cells was observed in the mice immunized with AdB7-infected cells but not in those immunized with AdCL-infected cells.
Conclusion These results suggest that the B7-1 gene has therapeutic potential for immunotherapy against head and neck squamous cell carcinoma.
Author Affiliations: Department of Otolaryngology–Head and Neck Surgery, Graduate School of Medicine (Drs Hoshitani, Ishida, Otsuki, and Nibu), International Center for Medical Research and Treatment, School of Medicine (Dr Shirakawa), Kobe University, Kobe, Japan; and Division of Cell and Gene Therapy, Institute for Advanced Medical Science, Hyogo College of Medicine, Hyogo, Japan (Dr Gotoh).
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