This Article  • Full text  • PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Citing articles on Web of Science (11)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Topic Collections  • Laryngology/ Speech/ Language Pathology  • Neoplasms of Head & Neck  • Pathology of Head & Neck  • Alert me on articles by topic  Social Bookmarking   What's this?

Christine G. Gourin, MD; Zhong-Sheng Xia, MD; Yan Han; Adam M. French, BS; Ashli K. O’Rourke, MD; David J. Terris, MD; Bao-Ling Adam, PhD

Arch Otolaryngol Head Neck Surg. 2006;132:390-397.

Objectives  To analyze surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF-MS) protein profiles of patients with head and neck squamous cell carcinoma (HNSCC) and healthy controls and to determine the sensitivity and specificity of SELDI assay for HNSCC detection before and after treatment.

Design  Proteomic analysis and comparison of serum samples.

Setting  Tertiary care academic medical center.

Subjects  Seventy-eight patients with HNSCC and 68 healthy controls.

Main Outcome Measures  Serum samples were prospectively collected from 78 patients with HNSCC and 68 healthy control volunteers. SELDI-TOF-MS was performed on serum samples to identify protein peaks in the range of 0 to 100 kDa. Classification analysis of the spectral data was performed and used to classify the disease status of the patients.

Results  The SELDI-TOF-MS assay generated serum protein profiles ranging from 0 to 100 kDa. After background subtraction, mass calibration, and normalization, 545 protein peaks were identified. Classification tree analysis based on peak expression correctly classified patients with HNSCC with 82% sensitivity and 76% specificity. Subgroup analysis correctly classified 83% of oral cavity tumors, 81% of oropharyngeal tumors, and 88% of laryngeal tumors. Pretreatment and posttreatment samples were available from 12 patients, and the posttreatment samples were correctly classified in 86% of the patients at 3 months and 75% of the patients at 6 months.

Conclusions  Proteomic SELDI-TOF-MS analysis of serum protein profiles distinguishes patients with HNSCC from controls with a high degree of sensitivity and specificity. Further investigation into the clinical utility of this technology in HNSCC detection and surveillance is warranted.

Author Affiliations: Department of Otolaryngology–Head and Neck Surgery (Drs Gourin, O’Rourke, Terris, and Adam and Mr French), and Center for Biotechnology and Genomic Medicine (Drs Xia and Adam and Ms Han), Medical College of Georgia, Augusta.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Detection of Papillary Thyroid Carcinoma With Serum Protein Profile Analysis
Moretz et al.
Arch Otolaryngol Head Neck Surg 2008;134:198-202.
ABSTRACT | FULL TEXT  

Serum Protein Profile Analysis Following Definitive Treatment in Patients With Head and Neck Squamous Cell Carcinoma
Gourin et al.
Arch Otolaryngol Head Neck Surg 2007;133:1125-1130.
ABSTRACT | FULL TEXT