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In Vitro Enzymatic Treatment and Carbon Dioxide Laser Beam Irradiation of Morphologic Cartilage Specimens
Jiannis K. Hajiioannou, MD;
Antonios Nikolidakis, MD;
Irene Naumidi;
Emmanuel Helidonis, MD;
Giannis Tzanakakis, MD;
George A. Velegrakis, MD
Arch Otolaryngol Head Neck Surg. 2006;132:1363-1370.
Objectives To determine the role of the main cartilage components in the internal system of interlocked stresses and to clarify the effect of laser beam irradiation on cartilage.
Design Control and experimental series.
Subjects Rabbit ear cartilage.
Intervention Rabbit ear cartilage strips incubated in collagenase and hyaluronidase enzyme solutions for specific periods were examined, and the observed changes in shape, strength, and elasticity were recorded, as well as the effect of carbon dioxide laser irradiation. Laser-pretreated cartilage strips were also incubated in the enzyme solutions to determine whether the laser-provoked changes were susceptible to enzymatic action. All cartilage pieces were examined by light and electron microscopy.
Results Collagenase-treated cartilage strips gradually lost their interlocked stresses, while hyaluronidase-treated strips mostly maintained their shape and their physical characteristics. Hyaluronidase-incubated cartilage strips altered their shape when they were laser treated. Collagenase-treated cartilages did not modify their shape when they were laser treated. Laser-pretreated cartilage pieces lost their new form in collagenase solutions but kept their laser-evoked shape when put in hyaluronidase solutions.
Conclusion The macroscopic observations combined with light and electron microscopy findings argue for the distinct role of the collagen network in morphologic cartilage shape and tensile strength preservation and provide a probable mechanism of cartilage transformation owing to carbon dioxide laser irradiation.
Author Affiliations: Department of Otolaryngology, University Hospital of Crete (Drs Hajiioannou, Nikolidakis, Helidonis, and Velegrakis), and Vardinoyiannion Eye Institute and Departments of Ophthalmology (Ms Naumidi) and Histology and Anatomy (Dr Tzanakakis), School of Medicine, University of Crete, Heraklion, Greece.
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