This Article  • Full text  • PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Citing articles on ISI (3)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Topic Collections  • Pathology of Head & Neck  • Alert me on articles by topic

Biochemical Analysis of Secretions

Ryan C. Branski, MA; Vlad C. Sandulache, BS; Joseph E. Dohar, MD, MS; Patricia A. Hebda, PhD

Arch Otolaryngol Head Neck Surg. 2005;131:153-157.

Objectives  To build on work in laryngology and oral biology that suggests utility in the assay of secretions collected from wound sites as a predictive instrument to determine which infants will likely develop subglottic stenosis following endotracheal intubation and to document and describe the wound-healing process.

Design  Prospective, randomized design.

Intervention  Laser-induced subglottic injury was established in 3 rabbits. Secretions were collected from the subglottic region at 6 time points from days 4 to 21 following injury and from 4 uninjured control airways. The secretions were then subjected to enzyme-linked immunoassays for interleukin 1 and prostaglandin E2.

Subjects  Three adult New Zealand white rabbits.

Main Outcome Measures  Interleukin 1 and prostaglandin E2 levels.

Results  Measurable amounts of both mediators were obtained. Furthermore, different temporal patterns of expression were observed with interleukin 1, showing increased levels on days 4 to 18, and with prostaglandin E2, showing increased levels on days 7 to 18. These results concur with emerging data regarding the role of each mediator in the wound-healing process.

Conclusion  Although in its infancy, the analysis of secretions collected from the site of injury in the subglottis may have utility in the management of patients following intubation-related trauma.

Author Affiliations: Otolaryngology Wound Healing Laboratory, Department of Pediatric Otolaryngology, Children’s Hospital of Pittsburgh (Messrs Branski and Sandulache and Drs Dohar and Hebda); Communication Science and Disorders, School of Health and Rehabilitation Sciences (Mr Branski) and McGowan Institute for Regenerative Medicine (Mr Sandulache and Drs Dohar and Hebda), University of Pittsburgh; and Cellular and Molecular Pathology Program, Department of Pathology (Mr Sandulache and Dr Hebda) and Department of Cell Biology and Physiology (Dr Hebda), University of Pittsburgh School of Medicine, Pittsburgh, Pa.

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Acute and Chronic Changes in the Subglottis Induced by Graded Carbon Dioxide Laser Injury in the Rabbit Airway
Otteson et al.
Arch Otolaryngol Head Neck Surg 2008;134:694-702.
ABSTRACT | FULL TEXT  

Graded Carbon Dioxide Laser-Induced Subglottic Injury in the Rabbit Model
Chafin et al.
Arch Otolaryngol Head Neck Surg 2007;133:358-364.
ABSTRACT | FULL TEXT  

Elucidating the Role of Interleukin 1beta and Prostaglandin E2 in Upper Airway Mucosal Wound Healing
Sandulache et al.
Arch Otolaryngol Head Neck Surg 2007;133:365-374.
ABSTRACT | FULL TEXT