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Ho-Sheng Lin, MD; Gerald J. Berry, MD; Willard E. Fee, Jr, MD; David J. Terris, MD; Zijie Sun, PhD

Arch Otolaryngol Head Neck Surg. 2004;130:311-316.

Objective  To identify protein-tyrosine kinases (PTKs) that may be involved in the development and progression of head and neck squamous cell carcinoma (HNSCC).

Design  Messenger RNA from 7 HNSCC specimens was reverse transcribed to complementary DNA, and selective amplification of PTK complementary DNA was achieved using polymerase chain reaction (PCR) with degenerate PTK primers. The resulting PTK PCR products from these 7 HNSCC specimens were then cloned and randomly selected for sequencing. The PTKs that were represented multiple times in these randomly selected clones were selected as candidate PTKs that may be overexpressed in HNSCC. Antibodies against these candidate PTKs were then used for immunohistochemical studies on 8 other HNSCC specimens not used in the original selection of the candidate PTKs.

Results  Three known (EphA1, Brk, and Ron) and 2 novel (KIAA0728 and KIAA0279) PTKs were found to be highly expressed in the 7 HNSCC samples studied, based on the technique of reverse transcriptase–PCR with degenerate primers. Immunohistochemical studies with antibodies against the 3 known PTKs in 8 other HNSCC specimens not used in the previous reverse transcriptase–PCR reaction demonstrated overexpression of EphA1, Brk, and Ron in 12.5%, 37.5%, and 75% of these specimens.

Conclusions  In this study, we identified 5 PTKs that were overexpressed in HNSCC using a reverse transcriptase–PCR technique and confirmed the overexpression of 3 known PTKs in some of the 8 archival HNSCC specimens studied. Our finding suggests that the signaling pathways mediated through EphA1, Brk, and Ron may be involved in the development and progression of HNSCC.

From the Department of Otolaryngology/Head and Neck Surgery, Wayne State University, Detroit, Mich (Dr Lin); the Department of Pathology (Dr Berry) and the Division of Otolaryngology (Drs Fee and Sun), Stanford University Medical Center, Stanford, Calif; and the Department of Otolaryngology, Medical College of Georgia, Augusta (Dr Terris). The authors have no relevant financial interest in this article.

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