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Association Between p53 Gene Mutations and Tobacco and Alcohol Exposure in Laryngeal Squamous Cell Carcinoma
Domenica Ronchetti, PhD;
Cesare B. Neglia, MD;
Bruno M. Cesana, MD;
Nadia Carboni, BSc;
Antonino Neri, MD;
Giancarlo Pruneri, MD;
Lorenzo Pignataro, MD
Arch Otolaryngol Head Neck Surg. 2004;130:303-306.
Objectives To analyze the relationship between p53 gene mutations, tobacco smoke, and alcohol consumption in patients with laryngeal squamous cell carcinoma.
Design We analyzed p53 gene mutations in exons 5 through 8 by polymerase chain reaction–single-strand conformation polymorphism analysis in a cohort of 84 patients with laryngeal squamous cell carcinoma.
Setting University hospital.
Results p53 gene mutations were detected in 24 (28.6%) cases (95% confidence interval, 19.3%-39.5%), and the GC to TA transversion (33%) was the most common type of mutation (95% confidence interval, 15.6%-55.3%). Most mutations mapped to the p53 DNA-binding domain, which is necessary for the physiological activity of p53 as a tumor suppressor. A statistically significant association was found between p53 mutations and exposure to tobacco smoke (P = .001), which was the only variable significantly associated with p53 mutations in a multivariate model. The association with alcohol consumption was only at a borderline level of significance (P = .065).
Conclusion Our data document that a smoking habit is the only independent variable associated with an increased risk of p53 mutations in the laryngeal mucosa.
From the Department of Otolaryngology (Drs Ronchetti, Neglia, and Pignataro), the Epidemiology Unit (Dr Cesana), the Third Division of Internal Medicine (Ms Carboni), and the Laboratory of Experimental Hematology and Molecular Genetics (Dr Neri), Ospedale Maggiore–Istituto di Ricovero e Cura a Carattere Scientifico, University of Milan, and the Division of Pathology and Laboratory Medicine, European Institute of Oncology (Dr Pruneri), Milan, Italy. The authors have no relevant financial interest in this article.
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