Innate Immunity of the Sinonasal Cavity
Expression of Messenger RNA for Complement Cascade Components and Toll-like Receptors
Joseph VanderMeer, MD;
Quan Sha, PhD;
Andrew P. Lane, MD;
Robert P. Schleimer, PhD
Arch Otolaryngol Head Neck Surg. 2004;130:1374-1380.
Objective To study the expression of important elements of the innate immune responses in human sinonasal tissue to elucidate its potential role in mucosal inflammation.
Design We studied human sinonasal tissue from patients with chronic rhinosinusitis and an immortalized epithelial cell line to detect the expression of innate immune effectors and the responses of these cells to stimulation with compounds associated with pathogenic organisms.
Patients Nine individuals undergoing endoscopic sinus surgery for chronic rhinosinusitis.
Main Outcome Measures Expression of complement components and toll-like receptors.
Results We found detectable levels of messenger RNA for all toll-like receptors in human sinonasal tissue and in the BEAS-2B epithelial cell line. Expression of several components of the alternate pathway of complement (factors B, H, and I and properdin) was constitutively present in unstimulated BEAS-2B cells and was readily detectable in human sinonasal tissue. Stimulation of BEAS-2B cells with the toll-like receptor 3 ligand double-stranded RNA resulted in increased expression of messenger RNA for factors B and H but not for properdin or factor I.
Conclusions Toll-like receptors and the alternate pathway of complement are important components of innate immunity that are expressed in human sinonasal epithelium in vivo and in cultured airway epithelial cells in vitro. The expression of some of these components can be significantly induced by stimulation via toll-like receptors, and epithelial expression of components of innate immunity may play a role in inflammation in chronic rhinosinusitis.
Author Affiliations: Department of Otolaryngology–Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Md (Drs VanderMeer and Lane); Johns Hopkins Bayview Asthma and Allergy Center, Baltimore (Dr Sha); and Allergy-Immunology Division, Northwestern University Feinberg School of Medicine, Chicago, Ill (Dr Schleimer).
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
Factor H Binding to PspC of Streptococcus pneumoniae Increases Adherence to Human Cell Lines In Vitro and Enhances Invasion of Mouse Lungs In Vivo
Quin et al.
Infect. Immun. 2007;75:4082-4087.
ABSTRACT
| FULL TEXT
Constitutive and Inducible Expression of B7 Family of Ligands by Human Airway Epithelial Cells
Kim et al.
Am. J. Respir. Cell Mol. Bio. 2005;33:280-289.
ABSTRACT
| FULL TEXT
|
