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The Effect of Growth Factors on the Proliferation and Differentiation of Human Nasal Gland Cells
Tetsuro Kimura, MD;
Yuichi Majima, MD;
Yongqing Guo, MD;
Toshimichi Yoshida, MD
Arch Otolaryngol Head Neck Surg. 2002;128:578-582.
Objective To elucidate a mechanism of proliferation and differentiation of nasal
gland cells, we established a serum-free 3-dimensional culture system for
human nasal gland (HNG) cells and examined the effects of epidermal growth
factor, keratinocyte growth factor, and retinoic acid on proliferation and
differentiation of cultured HNG cells.
Materials and Methods Nasal polyps were obtained from patients undergoing endoscopic endonasal
sinus surgery. The HNG cells were cultured under a monolayer culture and transferred
to a collagen-embedded culture using RPMI 1640 medium containing transferrin,
insulin, hydrocortisone, retinoic acid, epidermal growth factor, and keratinocyte
growth factor. Cell growth was measured by bromodeoxyuridine incorporation
assays. To measure cell differentiation, the percentage of cells containing
secretory granules, which were stained with Alcian blue in cytoplasm, was
determined.
Results In the serum-free 3-dimensional culture, the HNG cells showed ductal
structures containing secretory products in a lumen. The addition of epidermal
growth factor promoted the proliferation of HNG cells in its optimal concentrations,
and keratinocyte growth factor also enhanced the proliferation of HNG cells.
Conversely, the differentiation of HNG cells was not dependent on epidermal
growth factor and keratinocyte growth factor. Retinoic acid suppressed the
proliferation, but promoted the differentiation of HNG cells.
Conclusion Our culture system could be useful for studying the effects of various
growth factors and cytokines on HNG proliferation and differentiation to better
understand the mechanisms of growth and morphogenesis of nasal glands.
From the Departments of Otorhinolaryngology (Drs Kimura, Majima, and
Guo) and Pathology (Dr Yoshida), Mie University School of Medicine, Tsu, Mie,
Japan.
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