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Effect of Blood Transfusion in an Experimental Sarcoma Model
Ho-Sheng Lin, MD;
Ravi N. Samy, MD;
Joanne Lum, BS;
Mary Jo Dorie, PhD;
David J. Terris, MD
Arch Otolaryngol Head Neck Surg. 2002;128:308-312.
Objective To study the effect of allogeneic, syngeneic, and autologous blood transfusion
on the growth rate of the KHT tumor in a C3H murine model.
Design Prospective, randomized, and controlled animal study.
Subjects Sixty-one C3H female mice.
Interventions The C3H female mice were implanted with 2 x 105 cells
of KHT, a murine sarcoma. Ten days later, 0.3 mL of blood was removed from
a retro-orbital site to simulate surgical blood loss. This blood loss was
replaced by blood transfusion through a tail vein with the use of allogeneic
(major histocompatibility complex incompatible), syngeneic (major histocompatibility
complex compatible), or autologous blood. Tumor growth was measured daily
for 14 days. The tumor growth curve for each of the animals was constructed
and the mean slope of growth calculated for each group.
Results There were statistically significant differences in tumor growth rate
(P = .001) when the allogeneic group (mean slope
= 0.232, n = 14), the syngeneic group (mean slope = 0.190, n = 17), and the
autologous group (mean slope = 0.202, n = 14) were compared. A t test confirmed that there was no significant difference in the tumor
growth rate between the groups transfused with syngeneic and autologous blood
(P = .26). However, the rate of tumor growth in the
allogeneic group was found to be significantly higher when independently compared
with the syngeneic group (P<.001) and the autologous
group (P = .02).
Conclusions In this experimental model of a solid murine sarcoma, allogeneic blood
transfusion was associated with an increased rate of tumor growth compared
with syngeneic and autologous blood transfusion, likely reflecting immunomodulatory
effects incurred by the introduction of major histocompatibility complex–incompatible
antigens.
From the Divisions of Otolaryngology/Head & Neck Surgery (Drs Lin,
Samy, and Terris and Ms Lum) and Radiation Biology/Radiation Oncology (Dr
Dorie), Stanford University Medical Center, Stanford, Calif.
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Arch Otolaryngol Head Neck Surg. 2002;128(3):332-334.
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