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Chronic Bacterial Rhinosinusitis
Description of a Mouse Model
Abraham Jacob, MD;
Brian T. Faddis, PhD;
Richard A. Chole, MD, PhD
Arch Otolaryngol Head Neck Surg. 2001;127:657-664.
Objectives To survey normal murine sinonasal anatomy and to create a mouse model
for chronic bacterial rhinosinusitis.
Design Anatomic, histologic, and pathophysiologic study displaying normal murine
sinonasal anatomy and surgically created unilateral sinonasal inflammation.
Subjects Twenty-one 6-week-old, male C57BL/6 mice.
Interventions Animals that underwent unilateral maxillary sinus ostial obstruction
using Merocel nasal packing, animals with unilateral Bacteroides
fragilis inoculation alone, and animals with both ostial obstruction
and bacterial inoculation were examined at 4 weeks for histologic evidence
of chronic sinonasal inflammation. Experimental interventions were compared
with contralateral control sinuses within each animal and with normal and
sham-operated controls.
Results Normal mouse paranasal sinuses include maxillary sinuses, ethmoid air
cells, and respiratory-type epithelium. In experimental animals, the lateral
maxillary sinus wall, nasal septum, and superior turbinelle of the maxillary
sinus were examined histologically. Epithelial thickening and disarray, goblet
cell hyperplasia, inflammatory infiltrates, and sinonasal fibrosis were present
in the experimental sinuses of animals packed with Merocel alone or Merocel
with bacterial inoculation. Changes seen with Merocel and bacteria were more
dramatic than those with Merocel alone. Sham-operated controls and sinuses
inoculated with bacteria alone did not differ significantly from the sinuses
of normal animals.
Conclusion Unilateral maxillary sinus ostial obstruction using Merocel nasal packing
along with B fragilis inoculation results in a persistent,
localized bacterial rhinosinusitis in mice.
From the Department of Otolaryngology–Head and Neck Surgery,
Washington University School of Medicine, St Louis, Mo.
Corresponding author and reprints: Abraham Jacob, MD, Department
of Otolaryngology–Head and Neck Surgery, Washington University School
of Medicine, 660 S Euclid, Campus Box 8115, St Louis, MO 63110 (e-mail: Entsurgn{at}cs.com).
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