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The Mucosal Invasion Model
A Novel In Vitro Model for Evaluating the Invasive Behavior of Mucocutaneous Malignancies
Eben L. Rosenthal, MD;
Mark K. Wax, MD;
Peter Anderson, MD;
Molly Kulecz-Martin, PhD
Arch Otolaryngol Head Neck Surg. 2001;127:1467-1470.
Background Prevention of regional and metastatic spread of cutaneous malignancies
requires understanding the physiologic mechanism of tumor cell invasion. In
vitro models are convenient for studying the in vitro invasive phenotype of
normal cells, tumor cell lines, or genetically altered cells in a 3-dimensional
matrix, but they should attempt to recapitulate the complex in vivo submucosal
environment. A new acellular extracellular matrix, porcine submucosal matrix
(PSM), is thought to accurately recapitulate the submucosal matrix. A novel
in vitro model using PSM to assess mucocutaneous tumor cell invasion was studied.
Methods The morphologic characteristics, growth, and invasive behavior of human
head and neck squamous cell carcinoma (UM-SCC-1, UM-SCC-5, UM-SCC-17B, and
OSC-19) cell lines were assessed on the PSM gel and compared with commonly
used in vitro invasion models (type I collagen and Matrigel matrices). The
invasive phenotype of canine kidney cells was also assessed on each matrix,
because this cell line is known to demonstrate a characteristic in vitro invasive
phenotype.
Results The PSM-supported head and neck squamous cell carcinoma tumor cell line
growth and single cell invasion were seen under stimulated conditions, similar
to type I collagen gels. The invasive phenotype of canine kidney cells behaved
similarly on PSM and collagen. Matrigel did not support growth well, and invasion
occurred only superficially in isolated areas.
Conclusion The PSM is a good in vitro model for assessment of pharmacologic and
genetic manipulations of head and neck squamous cell carcinoma tumor cell
lines and has several advantages over other commonly used matrices.
From the Division of Otolaryngology–Head and Neck Surgery, University
of Alabama at Birmingham (Dr Rosenthal); and Section of Head and Neck Oncology,
Department of Otolaryngology–Head and Neck Surgery (Drs Wax and Anderson)
and Department of Dermatology (Dr Kulecz-Martin), Oregon Health Sciences University,
Portland.
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Arch Otolaryngol Head Neck Surg. 2001;127(12):1521-1522.
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