This Article  • Full text  • PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on Web of Science (1)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Topic Collections  • General Rhinology  • Pulmonary Diseases  • Pulmonary Diseases, Other  • Liver/ Biliary Tract/ Pancreatic Diseases  • Genetics  • Genetic Disorders  • Alert me on articles by topic  Social Bookmarking   What's this?

Sergio Hassid, MD; Georges Choufani, MD; Christine Decaestecker, PhD; Carine Delbrouck, MD; Sandra Dawance, MD; Patricia Pelc, MD; Nathalie Nagy, MD; Herbert Kaltner, VetMD; Isabelle Salmon, MD; André Danguy, PhD; Hans-Joachim Gabius, PhD; Robert Kiss, PhD

Arch Otolaryngol Head Neck Surg. 2000;126:769-776.

Objective  To investigate whether cystic fibrosis (CF)–related nasal polyps exhibit significantly distinct glycohistochemical characteristics when compared with single vs massive nasal polyps obtained from patients without CF.

Design  Glycohistochemical characteristics were identified by means of 8 histochemical probes, including 5 plant lectins (peanut, gorse seed, wheat germ, Maackia amurensis, and Sambucus nigra agglutinins), 2 animal lectins (14- and 16-kd galectins), and 1 neoglycoprotein (exposing the Thomsen-Friedenreich antigen). The binding of the 8 glycohistochemical markers was determined by means of computer-assisted microscopy. For each probe, 3 quantitative parameters were computed: the labeling index, which describes the percentage of tissue area specifically stained by a given marker; the mean optical density, which reflects the staining intensity; and the concentrational heterogeneity, which characterizes the level of heterogeneity of the staining intensity.

Subjects  A series of 61 nasal mucosa specimens was analyzed, including 6 normal cases, 23 single and 18 massive polyposis cases without CF, and 14 nasal polyps associated with CF.

Results  Normal and polyposal nasal mucosa differed in terms of the amounts and linkage types of sialic acids (revealed by the wheat germ, M amurensis, and S nigra agglutinins) rather than the characteristics of galactoside expression (monitored with the peanut agglutinin and 2 animal galectins). In contrast, nasal polyps markedly differed between patients with and without CF with respect to galactoside expression (revealed by the peanut agglutinin and the 14-kd galectin) and the display of binding site(s) for the neoglycoprotein.

Conclusion  Normal and polyposal nasal mucosa differ essentially in sialic acid presentation, while nasal polyps from patients with CF have a higher level of various lectin-reactive galactoside residues than nasal polyps from those without CF.

From the Departments of Ear-Nose-Throat Surgery (Drs Hassid, Choufani, Delbrouck, and Dawance) and Pathology (Drs Nagy and Salmon), Erasmus University Hospital, and the Laboratory of Histopathology (Drs Decaestecker, Danguy, and Kiss), Université Libre de Bruxelles, Fonds National de la Recherche Scientifique (FNRS) (Drs Decaestecker and Kiss), and the Department of Ear-Nose-Throat Surgery, Brugmann Hospital (Dr Pelc), Brussels, Belgium; and the Institute of Physiological Chemistry, Ludwig-Maximilians-University, Munich, Germany (Drs Kaltner and Gabius).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?