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A Preliminary Report

Brian J. Broker, MD; Rita Chakrabarti, PhD; Thane Blynman, MD; John Roesler, MD; Marilene B. Wang, MD; Eri S. Srivatsan, PhD

Arch Otolaryngol Head Neck Surg. 1999;125:676-680.

Background  Fetal wounds can heal without any histological evidence of scarring. Fetal wounds lack the inflammatory infiltrate characteristic of adult wounds, and the fetal environment is not necessary for scarless healing to occur. Recent evidence suggests that fibroblasts are the main effector of scarless healing in fetal tissue. What has not been shown is what profile of growth factors the fibroblast uses to influence wound repair.

Objective  To determine the expression of growth factors (transforming growth factors 1, 2, and 3; acidic and basic fibroblast growth factors; keratinocyte growth factor; and platelet-derived growth factor AA, BB, and AB) of fetal and adult fibroblasts in vitro.

Design  Adult and fetal fibroblasts were grown in culture, and messenger RNA was extracted by standard techniques. Northern hybridization was used to identify messenger RNA transcripts for the aforementioned growth factors. Densitometry was used to compare growth factor messenger RNA expression with that of a ubiquitously expressed control, glyceraldehyde phosphate dehydrogenase.

Results  The data suggest that fetal and adult fibroblasts express acidic and basic fibroblast growth factor and transforming growth factor 1. Adult fibroblasts show twice the relative expression of these growth factors compared with fetal fibroblasts.

Conclusions  The adult fibroblasts demonstrate a relative excess production of cytokines compared with fetal fibroblasts. This is thought to contribute to suboptimal wound healing in adult wounds compared with the scarless healing of fetal wounds.

From the Section of Facial Plastic Surgery, Division of Head and Neck Surgery, UCLA School of Medicine (Drs Broker, Wang, and Roesler), and Department of Surgery, UCLA School of Medicine and West Los Angeles Veterans Affairs Medical Center (Drs Chakrabarti, Blynman, Wang, and Srivatsan), Los Angeles, Calif. Dr Broker is now with the Institute for Applied Laser Surgery, Bala Cynwyd, Pa.

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