This Article  • Full text  • PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citation map  • Citing articles on HighWire  • Citing articles on ISI (12)  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

Wesley Hicks, Jr, MD; Lynn Sigurdson, PhD; Edward Gabalski, MD; Robert Hard, PhD; Leon Hall III, BSc; Joseph Gardella, PhD; Colin Powers, MD; Niranjan Kumar, PhD; Jamson Lwebuga-Mukasa, MD, PhD

Arch Otolaryngol Head Neck Surg. 1999;125:1239-1243.

Background  Maintaining tracheal integrity and restoring normal physiologic function after injury is complex. Some of the critical events in this process are deposition of a provisional extracellular matrix, tissue remodeling, and angiogenesis. These events are coordinated with epithelial migration and proliferation to restore the mucosal barrier. The ability of respiratory epithelial cells (REC) to migrate and proliferate and restore denuded areas of the large conducting airway after injury is poor.

Objective  To test the hypotheses that (1) the cartilaginous framework, underlying the extracellular matrix (submucosa) and epithelium, decreases the migratory ability of REC when compared with REC on the same provisional extracellular matrix (type I collagen) alone, and (2) this phenomenon is associated with a change in expression of transforming growth factor (TGF)- and TGF-, both of which have been demonstrated in cutaneous models to be important in epithelial migration and proliferation.

Design  We developed a culture system that reconstitutes the tracheal lumen in vitro, consisting of dissociated chondrocytes cultured in a manner to form cartilage, submucosa (type I collagen), and REC (termed a "composite culture"). Control cultures consisted of epithelial cells grown on type I collagen alone. Control and composite cultures were evaluated morphologically using scanning electron and light microscopy. Expression of TGF- and TGF- was determined in day 14 cultured epithelial cells from control and composite cultures by semiquantitative polymerase chain reaction.

Results  Epithelial cells from composite cultures did not spread and were less squamoid in morphological appearance than epithelial cells on type I collagen alone. Expression of both growth factors was reduced in epithelial cells from composite cultures compared with those on type I collagen.

Conclusions  Cartilage modulates TGF- and TGF- expression in REC, and may contribute to regulation of REC proliferation and differentiation.

From the Department of Head and Neck Surgery, Roswell Park Cancer Institute (Drs Hicks and Sigurdson and Mr Hall), Departments of Otolaryngology (Dr Gabalski), Anatomy/Cell Biology (Dr Hard), Chemistry (Dr Gardella), and Surgery (Dr Powers), SUNY at Buffalo Medical School, and Department of Pulmonary and Critical Care Medicine, University of Buffalo (Drs Kumar and Lwebuga-Mukasa), Buffalo, NY; and Department of Otolaryngology, Stanford University Medical Center, Stanford, Calif (Dr Gabalski).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Tissue-engineered endothelial and epithelial implants differentially and synergistically regulate airway repair
Zani et al.
Proc. Natl. Acad. Sci. USA 2008;105:7046-7051.
ABSTRACT | FULL TEXT  

Keratinocyte Growth Factor and Autocrine Repair in Airway Epithelium
Hicks et al.
Arch Otolaryngol Head Neck Surg 2004;130:446-449.
ABSTRACT | FULL TEXT  

Long-term evaluation of the replacement of the trachea with an autologous aortic graft
Martinod et al.
Ann. Thorac. Surg. 2003;75:1572-1578.
ABSTRACT | FULL TEXT  

Possible Impedance of Luminal Reepithelialization by Tracheal Cartilage Metalloproteinases
Sigurdson et al.
Arch Otolaryngol Head Neck Surg 2003;129:197-200.
ABSTRACT | FULL TEXT