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Harumi Jyonouchi, MD; Sining Sun, DDS; Cynthia A. Kennedy, MD; Keith C. Kajander, DDS, PhD; Frank L. Rimell, MD

Arch Otolaryngol Head Neck Surg. 1998;124:767-772.

Objective  To evaluate distribution of IgG antibodies (Ab) in the airway, ear, and sinuses in association with inflammatory changes in a rabbit sinusitis model.

Design  We measured IgG Ab and lactate dehydrogenase levels in solutions from sinus, airway, and middle ear lavage and in serum, and determined interferon messenger RNA expression in sinus and ear mucosa at 1, 2, 3, and 4 weeks after inoculation with Bacteroides fragilis.

Subjects  Six rabbits at each time point; controls were untreated (n=5) and sham-operated rabbits at 2 and 4 weeks (n=4-5).

Intervention  Bacteroides fragilis was inoculated into the left maxillary sinus with ostium closed.

Results  IgG Ab was undetectable in all controls. IgG Ab (>50 µg/g protein) was present at 2, 3, and 4 weeks in most bilateral sinus lavage samples and in 2 of 6, 5 of 6, and 6 of 10 ear lavage samples at 2, 3, and 4 weeks, respectively, following inoculation. Inflammatory changes (histological and lactate dehydrogenase) were much greater in the inflamed sinus. IgG Ab (>50 µg/g protein) was also detected in most bronchoalveolar lavage samples after 2 weeks. Interferon mRNA was undetectable in all untreated and most sham-operated controls but was detected in the bilateral sinus mucosa at 1 to 2 weeks, and remained detectable up to 4 weeks in most rabbits. Serum IgG Ab levels positively correlated with those in lavage samples, with highest correlation with right sinus lavage IgG Ab levels (r=0.56, P<.001).

Conclusion  IgG Ab levels in the upper airway mucosa likely increase within 2 weeks following bacterial inoculation as a part of mucosal immune responses independent of tissue necrosis.

From the Departments of Pediatrics (Drs Jyonouchi and Sun) and Otolaryngology (Drs Kennedy and Rimell), School of Medicine, and the Department of Oral Science, School of Dentistry (Dr Kajander), University of Minnesota, Minneapolis.

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Arch Otolaryngol Head Neck Surg 2000;126:522-528.
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Interferon Gamma Levels in the Sinus, Ear, and Airway in a Rabbit Sinusitis Model Induced by Bacteroides Inoculation
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