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Osteopontin and Bone Sialoprotein Distribution at the Bone Graft Recipient Site
Per Alberius, DMD, MD, PhD;
Monica Gordh, DMD, PhD
Arch Otolaryngol Head Neck Surg. 1998;124:1382-1386.
Background The area in close proximity to a bone graft is subject to marked remodeling activity, which may dramatically affect graft survival.
Objective To specifically analyze the effects at the recipient bed–onlay graft interface.
Design In 22 adult Lewis rats, bicortical grafts were positioned below the temporal muscle and subperiosteally over the parietal bone. The recipient bone was left intact or ground to remove the external cortical layer, thereby exposing the graft to the osteopotent cells of the bone marrow. The rats were killed after 4 or 20 weeks. The outcome was assessed by routine histologic examination and immunohistochemical labeling for 2 bone matrix proteins, osteopontin and bone sialoprotein, which are involved in bone resorption and formation, respectively.
Results Placement of the grafts submuscularly or grinding of the outer cortical layer of the host bed increased recipient site resorption. Resorptive activity (labeling) was concentrated to a subzone below the surface of the recipient bone; neither the graft surface nor the interface soft tissues were labeled.
Conclusion The successive loss of skeletal contour after bone grafting, in many cases, may largely result from recipient site failure rather than graft size reduction.
From the Department of Plastic Surgery, University Hospital MAS (Dr Alberius), and the Department of Oral Surgery, Centre for Oral Health Sciences, Lund University (Dr Gordh), Malmö, Sweden.
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