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Expression of the Transforming Growth Factor Isoforms in Inflammatory Cells of Nasal Polyps

André Coste, MD; Jean-Pascal Lefaucheur, MD; Qiu-Pin Wang, MD; Emmanuelle Lesprit, MD; Françoise Poron; Roger Peynegre, MD, PhD; Estelle Escudier, MD, PhD

Arch Otolaryngol Head Neck Surg. 1998;124:1361-1366.

Objective  To determine the expression and the potential role of transforming growth factor (TGF-) in nasal polyposis.

Design  Comparison of TGF- expression between normal and inflammatory nasal mucosa and polyps; in inflammatory nasal polyps, characterization of the TGF- isoforms expression and their potential location in macrophages and eosinophils.

Setting  Patients and samples were selected at the Hôpital Intercommunal, Créteil, France, and immunohistochemistry and immunoblots were performed at the Institut National de la Santé et de la Recherche Médicale U296 (Université Paris XII, France).

Subjects  Nasal polyps and nasal mucosa were sampled in 21 patients during ethmoidectomy, and muscosa was sampled in 6 healthy patients during rhinoplasty.

Methods  Immunohistochemistry and Western blot analysis were performed using specific antibodies to TGF-_1-3, TGF-₁, TGF-_2,and TGF-₃ isoforms. Double labeling was also performed using anti–TGF-₁ antibody together with macrophages or eosinophil-specific antibodies.

Results  The expression of TGF-_1-3 was significantly higher in inflammatory nasal polyps than in inflammatory nasal mucosa and higher in inflammatory nasal mucosa than in nasal mucosa from healthy patients. Transforming growth factor ₁ was the main isoform detected in inflammatory nasal polyps, and it was present in numerous macrophages and in some eosinophils.

Conclusions  Transforming growth factor , mainly TGF-₁, is strongly expressed in inflammatory nasal mucosa, where it could be produced by macrophages and eosinophils. Transforming growth factor could induce epithelium and connective tissue modifications and therefore be involved in the pathogenesis of nasal polyposis.

From the Departments of Oto-Rhino-Laryngology and Head and Neck Surgery (Drs Coste, Wang, and Peynegre) and Physiology (Dr Lefaucheur), Hôpital Intercommunal and Centre Hospitalo-Universitaire Henri Mondor de Créteil, Institut National de la Santé et de la Recherche Médicale, Unite U 296, Faculty of Medicine (Drs Coste and Escudier), the Department of Pediatrics, Hôpital Intercommunal de Créteil (Dr Lesprit), and Groupe d'Etudes et de Recherche sur le Muscle et le Nerf, Faculty of Medicine (Ms Poron), Université Paris XII, Créteil; and the Department of Histology, University Hospital Pitié-Salpêtriére, Université Paris VI, Paris (Dr Escudier), France.

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