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Kelly Bomer, MD; Alan Brichta, PhD; Fuad Baroody, MD; Sue Boonlayangoor, MS; Xiantang Li, DVM, PhD; Robert M. Naclerio, MD

Arch Otolaryngol Head Neck Surg. 1998;124:1227-1232.

Objective  To develop a mouse model of acute bacterial rhinosinusitis.

Design  Study mice (C57BL6/J) were inoculated intranasally with Streptococcus pneumoniae, ATCC 49619 suspended in trypticase soy broth, and controls were inoculated with trypticase soy broth alone. After 2, 5, or 14 days, intranasal cultures were obtained and mice were killed. The sinuses were prepared for histological investigation.

Setting  Animal care facility at a tertiary, academic institution.

Method  The histological sections of the sinuses were examined in a blinded manner for the percentage of sinus cavity occupied by neutrophil clusters, and for the number of neutrophils per square millimeter of sinus mucosa.

Results  Infected mice killed at 5 days had significantly more sinus area occupied by neutrophil clusters, significantly more neutrophils within the mucosa, and significantly more S pneumoniae growth in the intranasal cultures compared with controls (15/15 vs 0/6; P<.01). The amount of inflammation had decreased at 2 weeks.

Conclusion  Streptococcus pneumoniae induces acute bacterial rhinosinusitis in C57BL6/J mice as measured by culture and influx of neutrophils, and can be used as a model of acute bacterial rhinosinusitis.

From the Section of Otolaryngology–Head and Neck Surgery and the Clinical Microbiology Laboratories, University of Chicago, Chicago, Ill.

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