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Jian Zuo, MD; Kyle E. Rarey, PhD

Arch Otolaryngol Head Neck Surg. 1997;123(4):373-376.

Abstract
Objective
To localize the expression of secretory phospholipase A₂ (sPLA₂) in the cochlear duct.

Design
By means of an immunocytochemistry technique that used rabbit anti-porcine pancreatic phospholipase A₂ antiserum, sPLA₂ sites were identified in vivo and in vitro in the adult mouse cochlear duct.

Subjects
Eight C57BL/6N normal adult male mice (11-12 weeks old) (6 mice for the in vivo study, 2 mice for the in vitro study).

Results
Different patterns of immunostaining for sPLA₂ were observed in tissues of the cochlear duct. High levels of sPLA₂ were detected in the following areas: the basal cells of the stria vascularis, the organ of Corti, the spiral ganglion, and the cochlear nerve; moderate levels of sPLA₂ were found in the suprastrial and poststrial portions of the spiral ligament; low levels of sPLA₂ were observed in the marginal and intermediate cells of the stria vascularis and the infrastrial portion of the spiral ligament. Immunoreactivity for sPLA₂ also was detected in cultured marginal cells of the stria vascularis and in fibrocytes of the spiral ligament.

Conclusions
Secretory phospholipase A₂ is present in mouse cochlear tissues and individual cultured cells. These results suggest that the levels of sPLA₂ expression in the cochlear duct are cell specific.

Arch Otolaryngol Head Neck Surg. 1997;123:373-376

Author Affiliations
From the Departments of Anatomy and Cell Biology (Drs Zuo and Rarey), Otolaryngology (Dr Rarey), and Pediatrics (Dr Zuo), University of Florida College of Medicine, Gainesville.