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A Three-Dimensional Collagen Gel Matrix Culture

Takao Yamaguchi, MD; Takemoto Shin, MD; Hajime Sugihara, MD

Arch Otolaryngol Head Neck Surg. 1996;122(6):649-654.

Abstract
Objective
To prepare an in vitro reconstruction of the porcine laryngeal mucosa, for use in cell biological investigations and as a model for the study of laryngeal disease.

Design
Using separately obtained epithelial cells and fibroblasts from porcine laryngeal mucosa, we reconstructed the laryngeal mucosa in a three-dimensional collagen gel matrix culture with air-liquid interface.

Subjects
Porcine larynges were from 6-month-old pigs obtained from a local abattoir.

Results
We successfully reconstructed the laryngeal mucosa in vitro, including epithelium and lamina propria. The epithelial cells showed five to eight cells in thickness and were well differentiated on the reconstructed lamina propria. The differentiation-specific cytokeratin was positive.

Conclusions
To our knowledge, this is the first report of a reconstruction of the laryngeal mucosa in a three-dimensional collagen gel matrix culture. Fibroblasts and air-liquid interface treatment exert a great influence on the proliferation and differentiation of the cultured epithelial cells. This culture system will help to provide an appropriate physiologic environment to study the differentiation and disease of the larynx.

(Arch Otolaryngol Head Neck Surg. 1996;122:649-654)

Author Affiliations
From the Departments of Pathology (Drs Yamaguchi and Sugihara) and Otolaryngology (Drs Yamaguchi and Shin), Saga (Japan) Medical School.

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