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Joel M. Bernstein, MD, PhD; James R. Yankaskas, MD

Arch Otolaryngol Head Neck Surg. 1994;120(9):993-996.

Abstract
Objective
To measure transepithelial bioelectric properties of cultured human nasal polyp and turbinate epithelial cells to test the hypothesis that nasal polyps have increased rates of ion transport.

Design
Cohort study.

Setting
Private referral center.

Patients
Individuals undergoing surgery for symptomatic nasal obstruction due to polyps caused by cystic fibrosis, nonatopic rhinosinusitis, or allergic rhinosinusitis.

Methods
Epithelial cells were removed from separated polyp and turbinate samples by protease disaggregation and cultured on permeable collagen matrix supports. Transepithelial potential difference and resistance were measured daily. At the time of maximal transepithelial potential difference, the cultures were mounted in modified Ussing chambers and exposed to a sodium-positive channel blocker (amiloride hydrochloride) and to selected chloride-negative channel agonists (isoproterenol bitartrate, adenosine triphosphate).

Outcome Measures
Maximal transepithelial potential difference, resistance, and equivalent short-circuit current. Bioelectric responses to amiloride, isoproterenol, and adenosine triphosphate.

Results
Polyp cultures had higher transepithelial potential difference and equivalent short-circuit current than turbinate cultures. The mediator responses were greater for polyp than for turbinate cultures.

Conclusions
Polyp epithelia have increased Na⁺ absorption and Cl^– permeability relative to turbinate epithelia. These results are consistent with the hypothesis that increased epithelial fluid absorption contributes to the development of nasal polyps.

(Arch Otolaryngol Head Neck Surg. 1994;120:993-996)

Author Affiliations
From the Department of Speech–Language Pathology and Audiology, State College of New York-Buffalo, Department of Otolaryngology and Pediatrics, State University of New York–Buffalo, Department of Communicative Disorders and Sciences, State University of New York–Buffalo, and Division of Infectious Diseases, Children's Hospital of Buffalo (Dr Bernstein); and the Division of Pulmonary Diseases, Department of Medicine, University of North Carolina–Chapel Hill (Dr Yankaskas).

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