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Zlatko P. Pavelic, MD, PhD; Joseph Reising, MD; Ljiljana Pavelic; Daniel J. Kelley, MD; Peter J. Stambrook, PhD; Jack L. Gluckman, MD

Arch Otolaryngol Head Neck Surg. 1993;119(7):753-757.

Abstract
• P-glycoprotein (Pgp), encoded by the multidrug-resistance gene (MDR1), is an active efflux pump for many structurally diverse lipophilic compounds. Using peroxidase-antiperoxidase immunohistochemistry technique and four anti-Pgp monoclonal antibodies directed against different epitopes of the molecule, we examined the distribution of Pgp in normal human tissues and squamous cell carcinomas of the head and neck. All four antibodies detected Pgp in bronchial cells, mammary ductal epithelium, gallbladder epithelium, epithelia of small and large intestine, bile canaliculi, dermal sweat glands, proximal tubules of kidney, endometrium, trophoblasts, adrenal gland, and capillaries of central nervous system, testis, and papillary dermis. Of the 23 head and neck squamous cell carcinomas, about 60% had detectable Pgp. It is possible that differences noticed between antibodies are due to cross-reactivity to proteins unrelated to MDR1. Care must be taken in interpreting staining results when only one or two monoclonal antibodies are used.

(Arch Otolaryngol Head Neck Surg. 1993;119:753-757)

Author Affiliations
From the Department of Otolaryngology—Head and Neck Surgery (Drs Pavelic, Kelley, and Gluckman and Ms Pavelic), Surgery (Dr Reising), and Anatomy and Cell Biology (Dr Stambrook), College of Medicine, University of Cincinnati (Ohio).

Footnotes
Accepted for publication January 22, 1993.

Presented at the Third International Conference on Head and Neck Cancer, San Francisco, Calif, July 26, 1992.

Reprint requests to Division of Molecular Oncology, Department of Otolaryngology—Head and Neck Surgery, College of Medicine, University of Cincinnati, Cincinnati, OH 45267-0528 (Dr Pavelic).

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