You are seeing this message because your Web browser does not support basic Web standards. Find out more about why this message is appearing and what you can do to make your experience on this site better.


ABOUT ARCHIVES
Advanced Search

Welcome   | My Account | E-mail Alerts | Access Rights | Sign In


  Vol. 119 No. 7, July 1993 TABLE OF CONTENTS
  Archives
  •  Online Features
  ORIGINAL ARTICLES
 This Article
 •References
 •Full text PDF
 • Reply to article
 •Send to a friend
 • Save in My Folder
 •Save to citation manager
 •Permissions
 Citing Articles
 •Citation map
 •Citing articles on HighWire
 •Contact me when this article is cited
 Related Content
 •Similar articles in this journal
 Social Bookmarking
  Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit Add to Technorati Add to Twitter What's this?

Detection of P-Glycoprotein With Four Monoclonal Antibodies in Normal and Tumor Tissues

Zlatko P. Pavelic, MD, PhD; Joseph Reising, MD; Ljiljana Pavelic; Daniel J. Kelley, MD; Peter J. Stambrook, PhD; Jack L. Gluckman, MD

Arch Otolaryngol Head Neck Surg. 1993;119(7):753-757.


Abstract

• P-glycoprotein (Pgp), encoded by the multidrug-resistance gene (MDR1), is an active efflux pump for many structurally diverse lipophilic compounds. Using peroxidase-antiperoxidase immunohistochemistry technique and four anti-Pgp monoclonal antibodies directed against different epitopes of the molecule, we examined the distribution of Pgp in normal human tissues and squamous cell carcinomas of the head and neck. All four antibodies detected Pgp in bronchial cells, mammary ductal epithelium, gallbladder epithelium, epithelia of small and large intestine, bile canaliculi, dermal sweat glands, proximal tubules of kidney, endometrium, trophoblasts, adrenal gland, and capillaries of central nervous system, testis, and papillary dermis. Of the 23 head and neck squamous cell carcinomas, about 60% had detectable Pgp. It is possible that differences noticed between antibodies are due to cross-reactivity to proteins unrelated to MDR1. Care must be taken in interpreting staining results when only one or two monoclonal antibodies are used.

(Arch Otolaryngol Head Neck Surg. 1993;119:753-757)



Author Affiliations

From the Department of Otolaryngology—Head and Neck Surgery (Drs Pavelic, Kelley, and Gluckman and Ms Pavelic), Surgery (Dr Reising), and Anatomy and Cell Biology (Dr Stambrook), College of Medicine, University of Cincinnati (Ohio).


Footnotes

Accepted for publication January 22, 1993.

Presented at the Third International Conference on Head and Neck Cancer, San Francisco, Calif, July 26, 1992.

Reprint requests to Division of Molecular Oncology, Department of Otolaryngology—Head and Neck Surgery, College of Medicine, University of Cincinnati, Cincinnati, OH 45267-0528 (Dr Pavelic).



Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

The Role of ATP Binding Cassette Transporters in Tissue Defense and Organ Regeneration
Huls et al.
J. Pharmacol. Exp. Ther. 2009;328:3-9.
ABSTRACT | FULL TEXT  

P-glycoprotein: So Many Ways to Turn It On
Callaghan et al.
J Clin Pharmacol 2008;48:365-378.
ABSTRACT | FULL TEXT  

The ATP-Binding Cassette Transporters and Their Implication in Drug Disposition: A Special Look at the Heart.
Couture et al.
Pharmacol. Rev. 2006;58:244-258.
ABSTRACT | FULL TEXT  

Renal Interaction Between Itraconazole and Cimetidine
Karyekar et al.
J Clin Pharmacol 2004;44:919-927.
ABSTRACT | FULL TEXT  

Biodistribution, Radiation Dose Estimates, and In Vivo Pgp Modulation Studies of 18F-Paclitaxel in Nonhuman Primates
Kurdziel et al.
JNM 2003;44:1330-1339.
ABSTRACT | FULL TEXT  





HOME | CURRENT ISSUE | PAST ISSUES | TOPIC COLLECTIONS | CME | SUBMIT | SUBSCRIBE | HELP
CONDITIONS OF USE | PRIVACY POLICY | CONTACT US | SITE MAP
 
© 1993 American Medical Association. All Rights Reserved.