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Retrieval of p53 Protein in Paraffin-Embedded Head and Neck Tumor Tissues
Zlatko P. Pavelic, MD, PhD;
Louis G. Portugal, MD;
Michael J. Gootee, MD;
Peter J. Stambrook, PhD;
Christopher Smith, MD;
Richard E. Mugge;
Ljiljana Pavelic;
Keith Wilson, MD;
C. Ralph Buncher, ScD;
Ya-Qin Li, MD;
John S. McDonald, DDS, MS;
Jack L. Gluckman, MD
Arch Otolaryngol Head Neck Surg. 1993;119(11):1206-1209.
Abstract
Objectives
To improve the detection of p53 protein in formalin-fixed, paraffin-embedded head and neck tumor tissues.
Design
Cohort.
Setting:
University and Veterans Administration medical centers.
Patients or Other Participants
Retrospective samples.
Intervention
Surgery for head and neck carcinoma.
Main Outcome Measure
Retrieval of p53 antigen. Hypothesis formulated after data collection.
Results
An antigen retrieval method facilitated the unmasking of previously inaccessible p53 antigenic determinants in formalin-fixed, paraffin-embedded tissues. This approach has made possible a much more reliable and sensitive immunohistochemical detection of p53 antigen. The procedure is simple, requiring only microwave heating of tissue sections to 100°C in the presence of a zinc sulfate solution.
Conclusions
Antigen retrieval method in formalinfixed, paraffin-embedded tissue demonstrated a significant increase in p53 immunostaining.
(Arch Otolaryngol Head Neck Surg. 1993;119:1206-1209)
Author Affiliations
From the Departments of Otolaryngology—Head and Neck Surgery (Drs Pavelic, Portugal, Gootee, Smith, Wilson, Li, McDonald, and Gluckman, and Mr Mugge and Ms Pavelic), Anatomy and Cell Biology (Dr Stambrook), and Environmental Health (Dr Buncher), College of Medicine, University of Cincinnati (Ohio).
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