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The Analysis of Natural Killer Cell Activity by Flow Cytometry
Tamas Racz, MD;
Peter Sacks, PhD;
Nguyan T. Van, PhD;
Dorothy L. Taylor, MS;
Greg Young;
Samuel Bugis, MD;
Howard E. Savage, PhD;
Stimson P. Schantz, MD
Arch Otolaryngol Head Neck Surg. 1990;116(4):440-446.
Abstract
• A flow cytometric assay was used to detect the lytic and binding capacities of both fresh peripheral blood lymphocytes and purified Leu-19+ natural killer cells against head and neck cancer cell lines. Results demonstrated that natural killer cell-mediated cytotoxicity and effector-target conjugate formation evaluated by flow cytometry was significantly correlated with the standard chromium 51 release assay and the single-cell microscopic assay, respectively. The sorted Leu-19+ natural killer cells demonstrated higher lytic capacity with a corresponding higher binding rate compared with the unsorted peripheral blood lymphocytes and sorted Leu-19– cells. Flow cytometric analysis of natural killer cell activity (a rapid, simple, and quantifiable procedure) is an alternative to the standard chromium 51 release assay.
(Arch Otolaryngol Head Neck Surg. 1990;116:440-446)
Author Affiliations
From the Department of Head and Neck Surgery, National Institute of Oncology, Budapest, Hungary (Dr Racz); the Departments of Head and Neck Surgery (Drs Sacks, Savage, and Schantz, Ms Taylor, and Mr Young) and Hematology (Dr Van), The University of Texas MD Anderson Cancer Center, Houston; and the Department of Surgery, Queen's University, Hotel Dieu Hospital, Kingston, Canada (Dr Bugis).
Footnotes
Accepted for publication December 1, 1989.
Reprint requests to the Department of Head and Neck Surgery, Box 69, MD Anderson Cancer Center, 1515 Holcombe Blvd, Houston, TX 77030 (Dr Schantz).
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ABSTRACT
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