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Tumor Necrosis Factor and Cytotoxic AgentsEffect on Squamous Carcinoma Lines
Markus Gapany, MD;
Douglas E. Dawson, MD;
Christopher Schriever;
Robert Burgess, MD;
J. Randy Gipple;
Charles E. Riggs, Jr, MD
Arch Otolaryngol Head Neck Surg. 1990;116(4):436-439.
Abstract
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• The cytotoxicity of dactinomycin (actinomycin D), doxorubicin hydrochloride (Adriamycin), cisplatin, fluorouracil, and methotrexate alone and in combination with recombinant human tumor necrosis factor (rHuTNF) on human squamous cell carcinoma lines was studied by an MTT proliferation assay. The rHuTNF alone caused no inhibition after 24 to 72 hours. All lines investigated showed a dose-dependent response to dactinomycin and doxorubicin. Potentiation of dactinomycin and doxorubicin cytotoxicity occurred with four of six cell lines following incubation of rHuTNF and the drug. No synergistic effect on cytotoxicity was seen with rHuTNF and any chemotherapeutic agent on two cell lines. The addition of rHuTNF did not augment the cytotoxic effect seen with cisplatin, methotrexate, or fluorouracil on any cell line. These results show that rHuTNF can enhance the cytotoxic effect of certain chemotherapeutic agents on squamous cell lines in vitro.
(Arch Otolaryngol Head Neck Surg. 1990;116:436-439)
Author Affiliations
From the Departments of Otolaryngology Head and Neck Surgery (Drs Gapany, Dawson, and Burgess and Messrs Schriever and Gipple) and Internal Medicine, Division of Hematology and Oncology (Dr Riggs), The University of Iowa Hospitals and Clinics, Iowa City.
Footnotes
Accepted for publication September 19, 1989.
Reprint requests to Department of Otolaryngology Head and Neck Surgery, University of Iowa Hospitals and Clinics, Iowa City, IA 52242 (Dr Dawson).
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