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Human Medullary Thyroid CarcinomaInitial Characterization and In Vitro Differentiation of Two New Cell Lines
Elliot Abemayor, MD, PhD;
Neil Sidell, PhD;
Guy Juillard, MD
Arch Otolaryngol Head Neck Surg. 1989;115(4):478-483.
Abstract
• Medullary carcinoma of the thyroid (MCT), a tumor of calcitonin (CT)—secreting C cells, can display a variable malignant potential with poor prognosis linked to decreased cell differentiation. In vitro study of MCT has been hampered by the fact that few cell lines derived from this neoplasm have been available for study. Herein are reported the characteristics of two new lines derived from human MCT that are tumorigenic in athymic mice and do not secrete CT. After treatment with various concentrations of retinoic acid (a vitamin A derivative) and cyclic adenosine monophosphate, both lines exhibit the traits of more differentiated cells with a decrease in cellular proliferation and an increase in cytoplasmic CT content as shown by in situ immunoperoxidase staining. These cell lines should prove of great value in the study of the biology of MCT and the mechanisms underlying induced differentiation in this type of tumor.
(Arch Otolaryngol Head Neck Surg 1989;115:478-483)
Author Affiliations
From the Divisions of Head and Neck Surgery (Dr Abemayor), Neuropathology (Dr Sidell), and Radiation Therapy (Dr Juillard), UCLA School of Medicine; the Jonsson Comprehensive Cancer Center (Drs Abemayor, Sidell, and Juillard); and Harbor-UCLA Medical Center (Dr Abemayor), Los Angeles.
Footnotes
Accepted for publication Sept 21, 1988.
Read in part before the Second International Conference on Head and Neck Oncology, Arlington, Va, Oct 26, 1987.
Reprint requests to Division of Head and Neck Surgery, CHS 62-132, UCLA School of Medicine, Los Angeles, CA 90024 (Dr Abemayor).
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