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A New Technique for Treatment of Superficial Malignancies

Dan J. Castro, MD; Romaine E. Saxton, PhD; Harold R. Fetterman, PhD; Donna J. Castro, CST; Paul H. Ward, MD

Arch Otolaryngol Head Neck Surg. 1987;113(11):1176-1182.

Abstract
• Rhodamine 123 (Rh 123), a mitochondrial-specific dye with an absorption maxima at 511 nm, was tested as a new chemosensitizing agent for argon laser treatment of P₃ human squamous carcinoma cells. After exposure of P₃ cells in vitro to Rh 123 at doses of 1, 3, 6, and 10 µg/mL for one hour, we observed significant inhibition of DNA synthesis, except at the lowest dose. Rhodamine 123 at 1 µg/mL was selected to sensitize P₃ tumor cells for treatment with the monochromatic argon laser at 514.5 nm. Exposure of P₃ cells to laser energy levels of 700 to 950 J/cm² (36°C to 40°C after sensitization with Rh 123 completely inhibited tumor development of the P₃ cells transplanted subcutaneously into nude mice. Control P₃ cells treated with the laser alone at 36°C to 40°C or only with Rh 123 formed visible tumors by one week and continued to grow for the entire-week observation period. These results show that Rh 123 is a highly sensitive new fluorochrome for argon laser phototherapy of human squamous carcinoma cells.

(Arch Otolaryngol Head Neck Surg 1987;113:1176-1182)

Author Affiliations
From the Division of Head and Neck Surgery (Otolaryngology) (Drs Castro and Ward) and High-Frequency High-Speed Electronics Center (Dr Fetterman), UCLA School of Medicine, Division of Surgical Oncology, Veterans Administration Medical Center, Sepulveda, Calif (Dr Saxton), and the California Paramedical and Technical College, Long Beach, Calif (Ms Castro).

Footnotes
Accepted for publication June 18, 1987.

Read before the American Society for Head and Neck Surgery, Denver, April 30, 1987; read before the Association Research Otolaryngology Mid-Winter Meeting, Clearwater Beach, Fla, Feb 1, 1987.

Reprint requests to Division of Head and Neck Surgery, UCLA School of Medicine, Los Angeles, CA 90024 (Dr Ward).

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