This Article  • References  • Full text PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citing articles on HighWire  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

André B. M. Klaassen, PhD; Wim Kuijpers, PhD; Huub M. E. Scheres, MSc; Jaap F. Rodrigues de Miranda, PhD; Arie J. Beld, PhD

Arch Otolaryngol Head Neck Surg. 1986;112(4):428-431.

Abstract
• Radioligand receptor binding might give more detailed information on the innervation pattern of the nasal mucosa and the character of the various neuroreceptors involved. With respect to the cholinergic receptors, this technique reveals that specific binding of tritiated l-quinuclidinyl benzilate to rat nasal mucosa homogenates occurs to a homogeneous class of binding sites, with a dissociation constant of 0.06 ± 0.02nM and a receptor density of 8 ± 2 pmole/g of tissue. Binding is stereoselectively inhibited by benzetimide hydrochloride enantiomers. Pirenzepine displacement (inhibition constant = 0.5 x 10^–6M) classifies tritiated l-quinuclidinyl benzilate binding sites as M2-muscarinic receptors. Methylfurthrethonium inhibits tritiated l-quinuclidinyl benzilate binding at high concentrations, pointing to the presence of low-affinity agonist binding sites, probably admixed with a small proportion of high-affinity agonist binding sites. These data obtained in the rat open new perspectives for studying muscarinic receptors in the human nose to elucidate the supposed disturbance of autonomic nerve regulation in nasal hyperreactivity.

(Arch Otolaryngol Head Neck Surg 1986;112:428-431)

Author Affiliations
From the Departments of Otorhinolaryngology (Drs Klaassen and Kuijpers and Mr Scheres) and Pharmacology (Drs Rodrigues de Miranda and Beld), University of Nijmegen, the Netherlands.

Footnotes
Accepted for publication July 2, 1985.

Reprint requests to Department of Otorhinolaryngology, St Radboud Hospital, University of Nijmegen, Philips van Leydenlaan 15, 6500 HB Nijmegen, the Netherlands (Dr Klaassen).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Restrained whole body plethysmography for measure of strain-specific and allergen-induced airway responsiveness in conscious mice
Lofgren et al.
J. Appl. Physiol. 2006;101:1495-1505.
ABSTRACT | FULL TEXT