This Article  • References  • Full text PDF  • Reply to article  • Send to a friend  • Save in My Folder  • Save to citation manager  • Permissions  Citing Articles  • Citing articles on HighWire  • Contact me when this article is cited  Related Content  • Similar articles in this journal  Social Bookmarking   What's this?

David M. Harris, PhD; James H. Hill, MD; Jay A. Werkhaven, MD; Edward L. Applebaum, MD; Rocco V. Lobraico, MD; Stephen M. Waldow, PhD

Arch Otolaryngol Head Neck Surg. 1986;112(11):1194-1199.

Abstract
• Indistinct margin demarcation and autofluorescence are problems in fluorescence delineation of porphyrin-containing head and neck squamous cell cancer (HNSCC). We studied the time course and in vivo localization of porphyrin fluorescence in a squamous cell cancer hamster model and in human HNSCC. After intravenous injection, a gradient in fluorescence intensity developed rapidly until tumors fluoresced above a lower-intensity mucosal background. Hamster tumor and ulcerated HNSCC without porphyrin injection demonstrated autofluorescence grossly indistinguishable from fluorescence in porphyrin-injected tumors. However, fluorescence microscopy revealed autofluorescence to be a surface phenomenon and showed injected porphyrin localized in tumor stroma. We conclude that autofluorescence must be considered when interpreting porphyrin fluorescence. In addition, empirically designed photodynamic therapy can be effective in selected HNSCC. Data from animal experiments provide useful guidelines for the delivery of this therapy.

(Arch Otolaryngol Head Neck Surg 1986;112:1194-1199)

Author Affiliations
From the Department of Otolaryngology-Head and Neck Surgery, University of Illinois College of Medicine at Chicago (Drs Harris, Hill, Werkhaven, and Applebaum); the Wenske Laser Center, Ravenswood Hospital Medical Center, Chicago (Drs Harris, Hill, Lobraico, and Waldow); and the Department of Surgery, West Side Veterans Administration Medical Center, Chicago (Drs Hill and Applebaum).

Footnotes
Accepted for publication July 13, 1986.

Read before the American Society for Head and Neck Surgery, Palm Beach, Fla, May 8, 1986, and the American Society for Laser Medicine and Surgery, Orlando, Fla, May 29, 1985.

Reprint requests to Department of Otolaryngology-Head and Neck Surgery, University of Illinois College of Medicine at Chicago, 1855 W Taylor St, Suite 2.42, Chicago, IL 60612 (Dr Hill).

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter     What's this?

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES

Photodynamic Therapy in the Treatment of Squamous Cell Carcinoma of the Head and Neck
Wenig et al.
Arch Otolaryngol Head Neck Surg 1990;116:1267-1270.
ABSTRACT  

Combination Effect of Hyperthermia and Photodynamic Therapy on Carcinoma
Matsumoto et al.
Arch Otolaryngol Head Neck Surg 1990;116:824-829.
ABSTRACT