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Comparison With Effects on the MCF-7 Breast Cancer Cell Line

Amnon Shapira, MD; Erkki Virolainen, MD; John J. Jameson, MD; Steven J. Ossakow, MD; Thomas E. Carey, PhD

Arch Otolaryngol Head Neck Surg. 1986;112(11):1151-1158.

Abstract
• Six laryngeal squamous cell carcinoma (SCC) cell lines and the MCF-7 breast cancer line, all of which contain estrogen and progesterone receptors, were tested for in vitro growth inhibition by the antiestrogen tamoxifen citrate. Cell line MCF-7 was more sensitive to growth retardation at 1 µmol/L of tamoxifen citrate, but SCC lines showed higher sensitivity than MCF-7 to cytotoxic effects at higher drug concentrations (7 to 10 µmol/L). A cytostatic level of growth inhibition was produced by 5 µmol/L tamoxifen citrate for both types of cell lines. When tamoxifen and estradiol were added to cultures simultaneously, partial reversal of growth inhibition was observed with MCF-7 but not with UM-SCC-5. All of the cell lines recovered from inhibition when tamoxifen was replaced with 0.1 µmol/L estradiol, but laryngeal SCC lines recovered equally well in estradiol-free medium, whereas MCF-7 recovered only partially in the absence of estradiol. These findings indicate that there are at least two tamoxifen receptor sites—one for which estradiol is a competitor and one for which it is not—and that tamoxifen reversibly blocks growth and may synchronize SCC cells.

(Arch Otolaryngol Head Neck Surg 1986;112:1151-1158)

Author Affiliations
From the Cancer Research Laboratory, Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor. Dr Shapira is now with the Department of Otolaryngology, Kaplan Hospital, Rehovot, Israel; Dr Virolainen is now with the Department of Otolaryngology, University of Turku (Finland) Central Hospital; Dr Jameson is now with the Department of Otolaryngology, Tufts University Medical Center, Boston; and Dr Ossakow is now with the Department of Otolaryngology, University of Miami.

Footnotes
Accepted for publication Aug 16, 1986.

Read before the American Society for Head and Neck Surgery, Palm Beach, Fla, May 8, 1986.

Reprint requests to Cancer Research Laboratory, 6020 Kresge Hearing Research Institute, Box 028, University of Michigan, 1301 E Ann St, Ann Arbor, MI 48109 (Dr Carey).

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