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Analysis by Flow Cytometry and Chromosome Enumeration

R. Arturo Roa, MD; Thomas E. Carey, PhD; Peter P. Passamani, MD; Jay H. Greenwood, MS; Sandra Hsu; Eric O. Ridings, MA; Donald R. Schwartz, MS; Gregory T. Wolf, MD; Jerry L. Hudson, PhD

Arch Otolaryngol. 1985;111(9):565-575.

Abstract
• Fifteen squamous cell carcinoma cell lines derived from nine patients were examined for DNA content by flow cytometry and chromosome counts. Using human peripheral blood leukocytes and nucleated trout and chicken red blood cells as standards, the DNA indexes of the squamous cell carcinoma cell lines were found to range from 1.1 to 3.3. The DNA content was a stable characteristic of individual cell lines in multiple passages over a seven-month period. Although flow cytometry could detect abnormal DNA content even in diploid tumor lines, the chromosome number correlated well with the DNA content by flow cytometry. In cases in which more than one cell line was established from the same patient, the individual cell lines were found to differ in their DNA content. The cell lines established from metastatic or recurrent tumors usually had a lower DNA content and chromosome number and exhibited a more aggressive in vitro growth pattern than the primary tumor or earlier recurrence. We hypothesize that "streamlined" and aggressive cell populations may evolve in vivo from more slowly growing hyperploid precursor tumor cell populations when in the course of random loss of DNA or chromosomes those that confer no growth advantage are lost, while those that do confer growth advantage are retained.

(Arch Otolaryngol 1985;111:565-575)

Author Affiliations
From the Cancer Research Laboratory (Drs Roa and Carey, Ms Hsu, and Messrs Ridings and Schwartz), Department of Otolaryngology and Head and Neck Surgery (Drs Passamani and Wolf), and the Cell Identification Center, Department of Pathology (Mr Greenwood and Dr Hudson), University of Michigan, Ann Arbor.; From the Cancer Research Laboratory, 6020 KHRI, Box 028, University of Michigan, 1301 E Ann St, Ann Arbor, MI 48109 (Dr Carey).

Footnotes
Accepted for publication July 2, 1985.

Read before the annual joint meeting of the American Society for Head and Neck Surgery and the Society of Head and Neck Surgeons, Cerromar Beach, Puerto Rico, May 7, 1985.

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ABSTRACT