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A Marker for Differentiation

Michael J. Kaplan, MD; Stacey E. Mills, MD; Robert H. Rice, PhD; Michael E. Johns, MD

Arch Otolaryngol. 1984;110(11):713-716.

Abstract
• Involucrin is a major structural subunit of the cross-linked protein envelope that encases keratin in maturing squamous cells. Intracytoplasmic involucrin is identifiable via immunoperoxidase techniques as these cells migrate from the basal layer to the more superficial layers of the stratified epithelium. Normal squamous epithelia and mildly dysplastic epithelia show uniform staining in the suprabasal and superficial layers of the mucosa but show no staining in the basal layer. Moderate to severe dysplasias and invasive carcinomas demonstrate irregular or focal staining in all three layers. Thirtythree microscopic samples from 27 glottic laryngeal biopsy specimens were reviewed. The histochemically abnormal differentiation identified via involucrin staining correlated with accepted histologic criteria for dysplasia. Involucrin staining may provide objective information to assist the pathologist in differentiating degrees of dysplasia in laryngeal biopsy specimens.

(Arch Otolaryngol 1984;110:713-716)

Author Affiliations
From the Department of Otolaryngology—Head and Neck Surgery (Drs Kaplan and Johns), and Pathology (Dr Mills), University of Virginia Medical Center, Charlottesville, and Harvard School of Public Health, Boston (Dr Rice). Dr Kaplan is now with the University of California, San Francisco, and Dr Johns is now with The Johns Hopkins Hospital, Baltimore.

Footnotes
Accepted for publication Aug 3, 1984.

Read before the American Society for Head and Neck Surgery, Palm Beach, Fla, May 9, 1984.

Reprint requests to the Department of Otolaryngology—Head and Neck Surgery, University of California, A-717, 400 Parnassus Ave, San Francisco, CA 94143 (Dr Kaplan).

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