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Dendritic Cell Identification in Head and Neck Lymphoid TissueNewly Recognized Cells Control T-Lymphocyte Functions
William J. Richtsmeier, MD, PhD;
William E. Bowers, PhD;
Charles A. Ellsworth, MD;
Kevin Sorge, MS;
Mary Berkowitz
Arch Otolaryngol. 1984;110(11):701-706.
Abstract
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• The physiologic measurements of a subpopulation of mononuclear cells derived from head and neck lymphoid tissues are similar to those of dendritic cells are described. Dendritic cells are a subpopulation of bone marrow-derived leukocytes that were originally identified in rodents and now described in man as having central control of T-lymphocyte functions. We describe a technique for the enrichment of dendritic cells obtained from tonsils utilizing a bovine serum albumin (BSA) gradient and note that they have the light and electron microscopic appearance of dendritic cells. The measured oxidative mitogenic response and interferon- production in complete leukocyte cultures was compared with BSA gradient-separated preparations. The denser cells, comprised mostly of normal appearing lymphocytes, would not undergo a mitogenic response nor produce normal amounts of interferon when stimulated unless the dendritic cell-rich, lessdense fraction, was added back. The dendritic cells derived from tonsils seem to behave as a potent accessory cell for these T-lymphocyte-associated functions.
(Arch Otolaryngol 1984;110:701-706)
Author Affiliations
From the Department of Otolaryngology (Dr Richtsmeier) and the Department of Laboratory, Division of Research (Drs Bowers and Ellsworth, Mr Sorge, and Ms Berkowitz), The Mary Imogene Bassett Hospital, Cooperstown, NY.
Footnotes
Accepted for publication Aug 3, 1984.
Read before the American Society for Head and Neck Surgery, Palm Beach, Fla, May 10, 1984.
Reprint requests to Department of Otolaryngology/Head and Neck Surgery, RL 30 BB 1165, University of Washington Hospital, Seattle, WA 98195 (Dr Richtsmeier).
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