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Establishment and Characterization of New Permanent Cell Lines

Charles J. Krause, MD; Thomas E. Carey, PhD; Russell W. Ott; Charles Hurbis; Kenneth D. McClatchey, MD; Joseph A. Regezi, DDS

Arch Otolaryngol. 1981;107(11):703-710.

Abstract
• Squamous cell carcinoma is the most common of human cancers, and yet because it is poorly represented by cultured cell lines, little is known about the characteristic cell biology and the cellsurface antigenic phenotypes of such tumors. To develop a continuously available source of squamous cell carcinoma for repeated and reproducible serologic analysis and for better understanding of its biologic characteristics, tissue culture methods and nude mice were used to establish new cell lines of squamous carcinoma. Special media, serum supplements from several sources, and methods of handling fresh tissue specimens were all examined as a means of improving the survival of tumor cell lines. Several new cell lines were established. Features characteristic of a squamous cell origin, eg, microvilli, desmosomes, tonofilaments, and the squamous cell differentiation antigen (pemphigus antigen), were found. The clinical course of disease in individual donor patients has been examined.

(Arch Otolaryngol 1981;107:703-710)

Author Affiliations
From the Departments of Otorhinolaryngology (Drs Krause and Carey), Microbiology and Immunology (Dr Carey), and Pathology (Dr McClatchey), University of Michigan Medical Center, Ann Arbor; the Department of Genetics, Stanford (Calif) University Medical School (Mr Ott); and the Cell and Molecular Biology Program (Mr Hurbis) and Department of Pathology and School of Dentistry (Dr Regezi), University of Michigan, Ann Arbor.

Footnotes
Accepted for publication May 7, 1981.

Read before the combined meeting of the Society of Head and Neck Surgeons and the American Society for Head and Neck Surgery, Phoenix, Ariz, March 11, 1981.

Reprint requests to Department of Otorhinolaryngology, University of Michigan Hospitals, Ann Arbor, MI 48109 (Dr Krause).

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