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A Technique Using Scanning Electron Microscopy

Frank A. Voelker, DVM; Celine M. Henderson; Alexander W. Macklin, DVM, PhD; Walter E. Tucker, DVM

Arch Otolaryngol. 1980;106(10):613-617.

Abstract
• The laboratory rat is the most commonly used species in safety evaluation of pharmaceutical compounds. However, surface preparations of the organ of Corti (OC) are difficult to perform in this species largely due to problems in removing the thick, bony otic capsule. A modified technique was developed using rapid decalcification to solve this problem. In addition, scanning electron microscopy was used to quantitate hair cell damage and/or loss after exposure of the OC by microdissection. It was found that hair cell loss and damage could be easily detected and quantified in rats given gentamicin sulfate.

(Arch Otolaryngol 106:613-617, 1980)

Author Affiliations
From the Department of Toxicology and Experimental Pathology, Burroughs Wellcome Co, Research Triangle Park, NC.

Footnotes
Accepted for publication Dec 6, 1979.

Reprint requests to Technical Information Department, Burroughs Wellcome Co, 3030 Cornwallis Rd, Research Triangle Park, NC 27709 (Dr Voelker).

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